I would like to use a Du Jardin colouring. The recipe is described in the book "Microscopie als hobby" [Dutch] .
Problem is that the dyes & chemicals are not all readily available.
Making Astra blue solution is quite possible for me. I can order that chemicals.
But the second part [acridine-chrysoidin] of the staining I don't see where I can order the chemicals [in The Netherlands]. Chrysoidin is available for sale. Acridine red [C.I. 45000] I can't find. Possibly acridine red is too toxic by today's standards ?
Is there other good working workflow with same results ?
Du Jardin colouring (Where buy chemicals)
Re: Du Jardin colouring (Where buy chemicals)
Acridine red is simply out of production in Europe and the US. You ma find some of it in China or India at quite horrible cost.
Example: https://in.carbanio.com/chemical/acridi ... ch-ug0x1n4
580 Euros plus shipping, customs and VAT for 10 grams.
Try rhodamine B as a substitute.
Example: https://in.carbanio.com/chemical/acridi ... ch-ug0x1n4
580 Euros plus shipping, customs and VAT for 10 grams.
Try rhodamine B as a substitute.
Re: Du Jardin colouring (Where buy chemicals)
Thanks for the tip ! This explains Acridine red is nowhere to be found. And that I have to go other route.
In a search for rhodamine B I found a wonderful good German website. A very nice thorough comparative study of different colorings. And provides alternatives to no longer available Acridine red.
The Müller SAC staining I found there seems to me a good alternative. The chemicals are available. And not unnecessarily toxic or very expensive (useful if I want to use the same recipe in my teaching lessons)
In a search for rhodamine B I found a wonderful good German website. A very nice thorough comparative study of different colorings. And provides alternatives to no longer available Acridine red.
The Müller SAC staining I found there seems to me a good alternative. The chemicals are available. And not unnecessarily toxic or very expensive (useful if I want to use the same recipe in my teaching lessons)