Assess imaging levels

[woyjwjl]

BHS Splan 10X

P1000190.jpg (125.08 KiB) Viewed 4442 times

Splan 40X

P1000191.jpg (74.65 KiB) Viewed 4442 times

HI 100X 185 Short tube

P1000193.jpg (114.72 KiB) Viewed 4442 times

[MicroBob]

What are you trying to tell us?

[BramHuntingNematodes]

i would take the levels up a little they are a touch dim

[Hobbyst46]

IMHO, the Splan 10X can deliver sharper images. And the BH2 provides Kohler illumination.

[woyjwjl]

What are you trying to tell us?

The novice is not confident and wants to ask if everything is normal?

[woyjwjl]

i would take the levels up a little they are a touch dim

touch dim

[woyjwjl]

IMHO, the Splan 10X can deliver sharper images. And the BH2 provides Kohler illumination.

Maybe it is a picture compression problem, the original picture is 4608X3456

[woyjwjl]

IMHO, the Splan 10X can deliver sharper images. And the BH2 provides Kohler illumination.

Maybe it is a picture compression problem, the original picture is 4608X3456

[MicroBob]

Hi Woyjwjl,
I would suggest to use the full uploadable size of 1024 pixels on the long side and upt to 500kB per photo. Most objects look better when presented without the black corners. In the second image the white balance is a little on the warm side. I would adjust brightness and contrast so that the bright parts are really white. When sizing down it often is useful to apply a little sharpening as the last step. For basic and quick image editing I use Irfan View. It takes just a few clicks to optimize an image like I suggested.

Bob

[photomicro]

It is difficult to judge...as we don't know what the specimen is like.

My take on it is that the slide isn't great...

[woyjwjl]

Hi Woyjwjl,
I would suggest to use the full uploadable size of 1024 pixels on the long side and upt to 500kB per photo. Most objects look better when presented without the black corners. In the second image the white balance is a little on the warm side. I would adjust brightness and contrast so that the bright parts are really white. When sizing down it often is useful to apply a little sharpening as the last step. For basic and quick image editing I use Irfan View. It takes just a few clicks to optimize an image like I suggested.

Bob

Hi Bob,

Thanks for the reply

[woyjwjl]

It is difficult to judge...as we don't know what the specimen is like.

My take on it is that the slide isn't great...

High-resolution specimens seem to be difficult to obtain

[woyjwjl]

It is difficult to judge...as we don't know what the specimen is like.

My take on it is that the slide isn't great...

High-resolution specimens seem to be difficult to obtain

[MicroBob]

The new version of your photo looks better for me. I took it and set white balance to white for the gaps, increased contrast a little and sharpend a little - how do you like it?

I don't know who buys ready made slides - are there institutions who are willing to pay real money like 5-10€ per slide? The amateur sometimes buys redy made slides together with a cheap beginner microscope and as he doesn't want to risk much money for the instrument he prefers cheap slides. Most advanced amateurs don't buy slides. They do observations where self made fresh slides are used or make permanent slides themselves. In earlier times things were explained by means of projection microscopes. Today one can connect a microscope camera to a display or beamer, but one can also easlily make great micro photos and simoly show these. So the mrket for high quality slids is small.

[woyjwjl]

Great modifications!

In China, the price of commodity slices is 10 to 20 yuan, of course, the low price leads to uneven quality, as a developing country, first solve the problem and then pursue extreme quality, it seems that it is always like this.....

I am a complete novice as a microscope enthusiast, I have learned a lot of great knowledge in this forum, and thank you again for such a warm netizen.

I started experimenting with observing the pond water using slides... Well, it seems that high-power objectives plus low numerical aperture condensers can also observe effects similar to phase difference, darkfield, DIC, diffraction? Phantom?, well, it's a magical new world.....

[MicroBob]

I started experimenting with observing the pond water using slides... Well, it seems that high-power objectives plus low numerical aperture condensers can also observe effects similar to phase difference, darkfield, DIC, diffraction? Phantom?, well, it's a magical new world.....

I would suggest to first set up proper bright field illumination as close as your microscope will allow. When you use a condenser of at least the same aperture as the objective, fully opened, and pull out one eyepiece and look into the tube while slowly closing the aperture you will see when the aperture blades just start to creep into the image. Then reinsert eyepiece and oserve. The image will have shallow depth of field, be a bit faint, low contrast and high resolution. When closing down further conrast will increase and resolution will gradually be reduced. This way you can find out where the ideal point is to be found. Low aperture illumination will give you a very contrasty image with artefacts, but when loking closer the resolution is gone. Better is to start with the optimal resolution setting and introduce olique illumination.

[woyjwjl]

[/quote]
Better is to start with the optimal resolution setting and introduce olique illumination.
[/quote]

BHS doesn't seem to have a oblique illumination kit?

[MicroBob]

Oblique illumination has not been offered ready made by the bigger manufacturers for many decades. But it is not difficult to setup oblique illumination yourself. It may be enough to hold a finger just below the condenser.

[woyjwjl]

Magical fingers


Maybe it's oblique illumination that affects DIC sales