Recipe for a Romanowsky stain solution?

[Raul]

Hi guys I want to make the solution using methylene blue and eosin. I am interested in the proportions to use and also other aspects like buffers or oxidants for m.b.. I know that I can buy a cheap Giemsa stain, but I want to explore the science behind it.

Thank you.

[Seb28]

1 http://www.powershow.com/view1/f351b-ZD ... esentation

2 http://www.immunologie-labor.com/cellma ... ain_06.pdf

3 http://link.springer.com/article/10.100 ... 267#page-1

4 http://apps.who.int/iris/bitstream/1066 ... B_86.1.pdf

[75RR]

If you haven't come across Walter Dioni's articles in microscopy-uk, you should definitely have a look.

http://www.mic-uk.info/cgi-bin/search.c ... m_cat[]=-1

[Raul]

Thank you Seb28, the last link had some good information on quantities. I will experiment and if those look fine I will post some pictures:).

[Raul]

Thank you 75RR, I did come across his name about some months ago but I totally forgot about him. Thanks for refreshing my memory.