Watson Microsystem 70 microscope

[Bugone]

Just a bout Kohler illuminater just tuned my microscope and getting a 2d effect. Judy wondering if is this the right effect for Kohler it's amazing to see well happy

[75RR]

Köhler is mainly about even illumination and resolution. It is important to follow the procedure to achieve Köhler exactly.

"The main advantages of Koehler illumination in image formation are:
• Bright and even illumination in the specimen plane and in the conjugate image plane. Even when illumination is provided by an irregular light source such as a lamp filament, illumination of the object is remarkably uniform across an extended area. Under these conditions of illumination, a given point in the specimen is illuminated by every point in the light source, and, conversely, a given point in the light source illuminates every point in the specimen.
• Positioning of two different sets of conjugate focal planes at specific locations along the optic axis of the microscope. This is a strict requirement for maximal spatial resolution and optimal image formation for a variety of optical modes. As we will see, focusing the stage and condenser positions the focal planes correctly, while adjusting the field and condenser diaphragms controls resolution and contrast. Once properly adjusted, it is easier to locate and correct faults such as dirt and bubbles that can degrade optical performance."

FUNDAMENTALS OF LIGHT MICROSCOPY AND ELECTRONIC IMAGING
Douglas B. Murphy

By the way, did you buy that microscope from Ian Walker?

http://www.microscopy-uk.org.uk/mag/art ... son70.html

[Bugone]

Köhler is mainly about even illumination and resolution. It is important to follow the procedure to achieve Köhler exactly.

"The main advantages of Koehler illumination in image formation are:
• Bright and even illumination in the specimen plane and in the conjugate image plane. Even when illumination is provided by an irregular light source such as a lamp filament, illumination of the object is remarkably uniform across an extended area. Under these conditions of illumination, a given point in the specimen is illuminated by every point in the light source, and, conversely, a given point in the light source illuminates every point in the specimen.
• Positioning of two different sets of conjugate focal planes at specific locations along the optic axis of the microscope. This is a strict requirement for maximal spatial resolution and optimal image formation for a variety of optical modes. As we will see, focusing the stage and condenser positions the focal planes correctly, while adjusting the field and condenser diaphragms controls resolution and contrast. Once properly adjusted, it is easier to locate and correct faults such as dirt and bubbles that can degrade optical performance."

FUNDAMENTALS OF LIGHT MICROSCOPY AND ELECTRONIC IMAGING
Douglas B. Murphy

By the way, did you buy that microscope from Ian Walker?

http://www.microscopy-uk.org.uk/mag/art ... son70.html

I got this same one at work at a uni where I work got the picture on line thank you 75rr

[75RR]

I got this same one at work at a uni where I work got the picture on line thank you 75rr

You can link to the article if someone were to ask about that type of microscope, but you should really only show images of yours.

[Bugone]

I got this same one at work at a uni where I work got the picture on line thank you 75rr

You can link to the article if someone were to ask about that type of microscope, but you should really only show images of yours.

thanks 75rr read the info you gave me and known to get the Kohler illmimater set up now all the pond life are all much clear then befor and more detail so easy to do and not out of focus on the out side of the lenes now much more light getting thruogh as well thank for your help