Use and cleaning of oil immersion lenses

Do you have any microscopy questions, which you are afraid to ask? This is your place.
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charlie
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Use and cleaning of oil immersion lenses

#1 Post by charlie » Wed Apr 20, 2016 6:27 pm

I can see it will be a long time before I trust myself to observe a Klaus Kemp slide! It sure looks easy to break stuff.

Any advice for me beyond what these links advise? (They seem to treat the cleaning a bit cavalierly.)

https://www.youtube.com/watch?v=bp17YFeseY0

https://www.youtube.com/watch?v=_DZXqPZ ... t1TS-CC3KA

https://www.youtube.com/watch?v=KfqQErL64cY

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75RR
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Re: Use and cleaning of oil immersion lenses

#2 Post by 75RR » Wed Apr 20, 2016 8:13 pm

Did not think much of any of the videos.

I would suggest going with what the Zeiss "The Clean Microscope" leaflet says: http://microscopy.duke.edu/downloads/Th ... scsope.pdf


As for observing diatoms on a Klaus Kemp slide, this is a paragraph from an email he sent me when discussing the use of high magnification objectives with a very small Working Distance:

"It seems likely that when using an oil immersion objective with a close working distance that it is easy to rack down the focus when searching for the Diatoms to press on the coverslip and dislodge the specimens, one way to avoid this is to first focus on the blue circle around the Diatoms, then go to the 9 o'clock on the ring and then scan across to the three o'clock position, during the transition you are now more likely to come across the Diatoms which will now be in focus."
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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charlie
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Location: Oregon, WI USA

Re: Use and cleaning of oil immersion lenses

#3 Post by charlie » Thu Apr 21, 2016 11:46 am

That is a great tip from Klaus Kemp! Thanks for sharing that.

It must have been memories from reading "The Clean Microscope" that made me cringe at the videos that were linked.

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