today's compound microscope

Do you have any microscopy questions, which you are afraid to ask? This is your place.
Post Reply
Message
Author
User avatar
MicroInspector
Posts: 365
Joined: Tue May 03, 2016 7:01 am

today's compound microscope

#1 Post by MicroInspector » Mon Jul 18, 2016 10:48 pm

A question to be asked
With today's modern microscopes can the common cold virus
can be seen with compound microscope ?
Or you will need a electron microscope?
The microscope reveals the truth of life.
Omax M82EZ microscope

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: today's compound microscope

#2 Post by gekko » Tue Jul 19, 2016 12:25 am

Whatever happened to the feather that you were going to show us a photomicrograph of?

User avatar
MicroInspector
Posts: 365
Joined: Tue May 03, 2016 7:01 am

Re: today's compound microscope

#3 Post by MicroInspector » Tue Jul 19, 2016 12:56 am

I am in the process of moving.
How ever I also got a little spider
Been thinking how to put it on the slide.
The microscope reveals the truth of life.
Omax M82EZ microscope

billbillt
Posts: 2895
Joined: Mon Nov 10, 2014 10:01 pm

Re: today's compound microscope

#4 Post by billbillt » Tue Jul 19, 2016 5:04 am

A virus is too small to see with a light microscope.. It requires an electron microscope.

User avatar
MicroInspector
Posts: 365
Joined: Tue May 03, 2016 7:01 am

Re: today's compound microscope

#5 Post by MicroInspector » Tue Jul 19, 2016 5:41 am

So Even Smaller Than Bacteria
The microscope reveals the truth of life.
Omax M82EZ microscope

billbillt
Posts: 2895
Joined: Mon Nov 10, 2014 10:01 pm

Re: today's compound microscope

#6 Post by billbillt » Tue Jul 19, 2016 5:49 am

yes

User avatar
MicroInspector
Posts: 365
Joined: Tue May 03, 2016 7:01 am

Re: today's compound microscope

#7 Post by MicroInspector » Tue Jul 19, 2016 6:00 am

I wonder how they make a virus culture and slice it?
The microscope reveals the truth of life.
Omax M82EZ microscope

billbillt
Posts: 2895
Joined: Mon Nov 10, 2014 10:01 pm

Re: today's compound microscope

#8 Post by billbillt » Tue Jul 19, 2016 6:15 am

i have never heard of a sliced virus.. a virus would not need to be sliced.. a TEM will see through it.

Tom Jones
Posts: 146
Joined: Wed Apr 13, 2016 3:47 pm

Re: today's compound microscope

#9 Post by Tom Jones » Fri Jul 22, 2016 3:42 pm

Viruses are cultured in living cells such as HeLa, or another cell line. The cells themselves, with the virus still inside, and/or the virus particles themselves after exiting the cells, are then embedded in some form of epoxy. The epoxy block is sliced very, very thin (less than 100 nm :shock: ) with an ultramicrotome and the sections stained to provide contrast with a material like osmium or uranyl acetate. Viruses will be sliced if they are in embedded material. If they are simply deposited on the grid (EM version of a slide) and stained, no sectioning is done so they will not be sliced.

User avatar
MicroInspector
Posts: 365
Joined: Tue May 03, 2016 7:01 am

Re: today's compound microscope

#10 Post by MicroInspector » Fri Jul 22, 2016 11:59 pm

Well that answers question
Has anyone seen the movie called The Andromeda Strain?
The microscope reveals the truth of life.
Omax M82EZ microscope

User avatar
mrsonchus
Posts: 3781
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: today's compound microscope

#11 Post by mrsonchus » Sat Jul 23, 2016 12:31 am

Life's too short.
John B

User avatar
MicroInspector
Posts: 365
Joined: Tue May 03, 2016 7:01 am

Re: today's compound microscope

#12 Post by MicroInspector » Sat Jul 23, 2016 1:02 am

:o what does that mean?
I would think you might saw that movie. ...
The microscope reveals the truth of life.
Omax M82EZ microscope

Oktagon
Posts: 180
Joined: Thu Apr 07, 2016 12:52 am

Re: today's compound microscope

#13 Post by Oktagon » Wed Jul 27, 2016 8:22 pm

What Tom Jones said.

You can also use SEM and look at cells full of viral particles to study topography. The only time you can use light microscopy to detect viruses (not observe them directly) is if you use florecently labeled antibody and if you have high viral load in your culture, you will see it using florecent or LCM scope.

Post Reply