Is this normal for objective lenses?

[Lilly Begonia]

I am finding that I get rather fuzzy resolution with my most powerful objective, 100/1.25, much less clear than with my 40/0.65, and I'm wondering if that normal. I'd like the image to be equally clear in both lenses.

[Charles]

Are using oil immersion with the 100X 1.25NA objective?

[Lilly Begonia]

Are using oil immersion with the 100X 1.25NA objective?

Well, no, I've not gotten to that yet, I'm quite new to this, will it help?

[Peter]

will it help?

Oh yes.

[Lilly Begonia]

will it help?

Oh yes.

Aha!!! I tried it and it works really great! Thank you! Another new thing learned! I'm assuming I'll have to clean the lens afterwards.

[Peter]

I'm assuming I'll have to clean the lens afterwards.

Oh yes.

[Lilly Begonia]

I'm assuming I'll have to clean the lens afterwards.

Oh yes.

Well this is truly groovy! I get lots of detail now! I had some icky water in the tray under my dish rack, and put a drop on a slide, put a slip cover over that, and it's just teeming with bacteria! Some of those little buggers race around so fast I can't get a look at them others look like maybe amoeba. There are lots of round bacteria, and some green round ones with a red cell wall around them.

Thanks for the hint Peter, may rainbows and unicorns fill your day

[carlh6902]

Lilly, just make sure you don't ever get oil on any of the other objectives. They can be ruined by the oil.

Carl