(Experience) Legume starch granules

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Pablo M
Posts: 2
Joined: Tue Feb 06, 2018 2:20 pm

(Experience) Legume starch granules

#1 Post by Pablo M » Tue Feb 06, 2018 2:39 pm

Hello Comunity!

My name is Pablo, I'm from Chile, and I'm wondering if you can help me out with this experience.
I am trying to observe legume starch from a flour already milled. I know that i can use a iodine solution to stain the starch granules, but im not really sure if a light microscope (an optical, thats all i have for now) can do the job. The final point is to observe structure, if theres starch rupture or not.

Any help would be appreciate it... btw the starch granules are in the range of 20-50 um.

Thanks!

MichaelG.
Posts: 4026
Joined: Mon Apr 10, 2017 8:24 am
Location: North Wales

Re: (Experience) Legume starch granules

#2 Post by MichaelG. » Tue Feb 06, 2018 3:03 pm

Welcome, Pablo

I think you will find this page useful:
http://www.micromagus.net/pharma/starch.html

Viewing through crossed polars is usually best.

MichaelG.
.
Edit: https://www.microscopyu.com/techniques/ ... microscopy
You don't need anything very technical ... two discs of Polaroid filter will do.
Too many 'projects'

Pablo M
Posts: 2
Joined: Tue Feb 06, 2018 2:20 pm

Re: (Experience) Legume starch granules

#3 Post by Pablo M » Tue Feb 06, 2018 4:04 pm

MichaelG. wrote:Welcome, Pablo

I think you will find this page useful:
http://www.micromagus.net/pharma/starch.html

Viewing through crossed polars is usually best.

MichaelG.
.
Edit: https://www.microscopyu.com/techniques/ ... microscopy
You don't need anything very technical ... two discs of Polaroid filter will do.
Thanks Michael, im already in to that. Any recommendation on the filters? what i got is a zeiss primo star, very basic i think, but for learning purpose is all that i need (for now).

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