Glycerin as a mounting medium
Glycerin as a mounting medium
I'm looking at Beginner Microscope Experiments on microscopemaster.com For the experiment looking at leaf structure, it call for glycerin as a mounting medium. Why? When is this the correct choice?
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Re: Glycerin as a mounting medium
Glycerine (UK spelling) , also referred to as glycerol, is used as a temporary mountant, in that it won't dry out very quickly, and (pure) has a refractive index similar to the glass slide.Maryfox wrote:I'm looking at Beginner Microscope Experiments on microscopemaster.com For the experiment looking at leaf structure, it call for glycerin as a mounting medium. Why? When is this the correct choice?
Glycerine/glycerol jelly is a permanent mountant, flows when warm and sets. It is miscible with water, and very easy to use.
Hope this helps.
Mike
Re: Glycerin as a mounting medium
Thanks, Mike. You've added to my meager knowledge.
Re: Glycerin as a mounting medium
Further to other information it helps if you warm all the equipment you use slides, coverglasses, dropping rods when making slides with glycerine jelly it reduces the risk of air bubbles. If you add a drop of formalin to the underside of the coverslip before placing it on the glycerine jelly it stops the jelly melting again when warmed. If you want to make the slide permanent it should be ringed. Lots of info out there to help. Good luck!
Re: Glycerin as a mounting medium
Serendipity !!
This reissue of a paper by Walter Dioni has appeared on micscape:
http://www.microscopy-uk.org.uk/mag/art ... sited.html
MichaelG.
This reissue of a paper by Walter Dioni has appeared on micscape:
http://www.microscopy-uk.org.uk/mag/art ... sited.html
MichaelG.
Too many 'projects'
Re: Glycerin as a mounting medium
Thanks, Michael Great info there.