Do it yourself double cover slips to enable observations from both sides?

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lperepol
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Do it yourself double cover slips to enable observations from both sides?

#1 Post by lperepol » Fri Jun 21, 2019 2:35 am

Anyone have techniques they can share for DIY (Do It Yourself) double cover slips to enable observations from both sides?

A DIY alternative to Cobb’s aluminium slide holders:
https://www.wur.nl/en/show/Cobbs-alumin ... ders-1.htm
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Re: Do it yourself double cover slips to enable observations from both sides?

#2 Post by 75RR » Fri Jun 21, 2019 5:24 am

Not heard of this (though I can see the usefulness of viewing the top and bottom of a specimen) but would have thought the problem to overcome would be the thickness of the slide.

With a normal slide one would be (on the underside) attempting to focus through 1mm of glass.
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Re: Do it yourself double cover slips to enable observations from both sides?

#3 Post by MichaelG. » Fri Jun 21, 2019 7:02 am

75RR wrote:Not heard of this (though I can see the usefulness of viewing the top and bottom of a specimen) but would have thought the problem to overcome would be the thickness of the slide.

With a normal slide one would be (on the underside) attempting to focus through 1mm of glass.
If I read the linked page correctly ... there is no glass slide involved
The design appears to be a perforated aluminium holder, for a specimen sandwiched between two slips.

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75RR
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Re: Do it yourself double cover slips to enable observations from both sides?

#4 Post by 75RR » Fri Jun 21, 2019 8:06 am

MichaelG. wrote: If I read the linked page correctly ... there is no glass slide involved
The design appears to be a perforated aluminium holder, for a specimen sandwiched between two slips.

MichaelG.
That makes more sense. Thanks
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Re: Do it yourself double cover slips to enable observations from both sides?

#5 Post by lperepol » Fri Jun 21, 2019 4:09 pm

Come to think of it they may be easy to make.
1) Drill a 20 mm hole in the center of a strip of aluminum about the size of a slide (size 25 mm 75 mm),
2) Center and mount with nail polish, or beeswax a coverslip over the hole.
3) Add specimen, dry mount another coverslip, over the specimen, sandwiching the specimen.

I'll have to try it out.
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Re: Do it yourself double cover slips to enable observations from both sides?

#6 Post by microb » Fri Jun 21, 2019 6:46 pm

Found this by accident. As for slides, one could 3D print as well.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411252/

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Re: Do it yourself double cover slips to enable observations from both sides?

#7 Post by ImperatorRex » Fri Jun 21, 2019 8:21 pm

I use 24x60mm cover glasses instead of object carrier glasses. Two of them make a perfekt sandwich that I put into into a frame made for the 76mm length to fit in any object carrier or stage.

Have a look pls.:
http://show.picr.de/32343749ju.jpg.html

I use it for the inverted microscope, but sometimes I use the same on the normal microscope.
I can use this setup even for oil immersion if I make the immersion on condensor and objective, bending is limited then.

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