Do it yourself double cover slips to enable observations from both sides?

[lperepol]

Anyone have techniques they can share for DIY (Do It Yourself) double cover slips to enable observations from both sides?

A DIY alternative to Cobb’s aluminium slide holders:
https://www.wur.nl/en/show/Cobbs-alumin ... ders-1.htm

[75RR]

Not heard of this (though I can see the usefulness of viewing the top and bottom of a specimen) but would have thought the problem to overcome would be the thickness of the slide.

With a normal slide one would be (on the underside) attempting to focus through 1mm of glass.

[MichaelG.]

Not heard of this (though I can see the usefulness of viewing the top and bottom of a specimen) but would have thought the problem to overcome would be the thickness of the slide.

With a normal slide one would be (on the underside) attempting to focus through 1mm of glass.

If I read the linked page correctly ... there is no glass slide involved
The design appears to be a perforated aluminium holder, for a specimen sandwiched between two slips.

MichaelG.

[75RR]

If I read the linked page correctly ... there is no glass slide involved
The design appears to be a perforated aluminium holder, for a specimen sandwiched between two slips.

MichaelG.

That makes more sense. Thanks

[lperepol]

Come to think of it they may be easy to make.
1) Drill a 20 mm hole in the center of a strip of aluminum about the size of a slide (size 25 mm 75 mm),
2) Center and mount with nail polish, or beeswax a coverslip over the hole.
3) Add specimen, dry mount another coverslip, over the specimen, sandwiching the specimen.

I'll have to try it out.

[microb]

Found this by accident. As for slides, one could 3D print as well.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411252/

[ImperatorRex]

I use 24x60mm cover glasses instead of object carrier glasses. Two of them make a perfekt sandwich that I put into into a frame made for the 76mm length to fit in any object carrier or stage.

Have a look pls.:
http://show.picr.de/32343749ju.jpg.html

I use it for the inverted microscope, but sometimes I use the same on the normal microscope.
I can use this setup even for oil immersion if I make the immersion on condensor and objective, bending is limited then.