Phase contrast objectives

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Cellusome
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Phase contrast objectives

#1 Post by Cellusome » Tue Jul 23, 2019 7:59 pm

Can phase contrast objectives be used for normal brightfield without a real downgrade in resolution? I was planning to buy a microscope only including the zernike phase contrast condenser, but I'd like to also have the option to view specimen in brightfield.

MichaelG.
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Re: Phase contrast objectives

#2 Post by MichaelG. » Tue Jul 23, 2019 8:14 pm

The downgrade in resolution is real ... but not generally sufficient to prohibit their use in non-phase conditions.

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Re: Phase contrast objectives

#3 Post by admin » Tue Jul 23, 2019 8:47 pm

The phase plate in the objective will reduce the resolution somewhat. But I think that there are other factors that can outweigh the loss of quality (dust, mounting medium etc).... A wrong condenser aperture setting will also reduce the resolution and I still often use it, because I get a larger depth of field, for example.

Oliver.
Science may set limits to knowledge, but should not set limits to imagination.
(Bertrand Russell)

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