Phase contrast objectives
Phase contrast objectives
Can phase contrast objectives be used for normal brightfield without a real downgrade in resolution? I was planning to buy a microscope only including the zernike phase contrast condenser, but I'd like to also have the option to view specimen in brightfield.
Re: Phase contrast objectives
The downgrade in resolution is real ... but not generally sufficient to prohibit their use in non-phase conditions.
MichaelG.
MichaelG.
Too many 'projects'
Re: Phase contrast objectives
The phase plate in the objective will reduce the resolution somewhat. But I think that there are other factors that can outweigh the loss of quality (dust, mounting medium etc).... A wrong condenser aperture setting will also reduce the resolution and I still often use it, because I get a larger depth of field, for example.
Oliver.
Oliver.
Science may set limits to knowledge, but should not set limits to imagination.
(Bertrand Russell)
(Bertrand Russell)