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immersion oil question
Posted: Sat Sep 07, 2019 12:24 am
by mintakax
I ask forgiveness io advance if this is a bit "over the top". I have a small pond aquarium and I have a procedure which, to my thinking, results in the least harm to the organisms I observe. I hope I'm not just fooling myself.
I use a well slide with a glass cover and after observing, I dunk the entire slide in the aquarium water until the cover slide floats off a bit. I lift it off with a pair of tweezers and rinse it in the aquarium, then toss it. I similarly rinse the slide, but re-use it.
I'm wondering if following the same procedure with a slide that had immersion oil (after being wiped off) on the cover would eventually foul up the aquarium water ? I know it will float on the surface, but the surface exchange with the air must be a pretty big deal.
Thanks,
Dan
Re: immersion oil question
Posted: Sat Sep 07, 2019 2:03 am
by wporter
I would think that the oil, especially if it eventually spreads out to form a sheen or layer, would probably negatively impact the air-water gaseous exchange rate or process.
Re: immersion oil question
Posted: Sat Sep 07, 2019 5:04 am
by MicroBob
This is probably no design criteria for the formulation of immersion oil so I would be careful.
You might look for a clear natural oil of suitable refractive index instead. This oil might not be perfect optically and it is not tested for use on microscope objectives but this may not be a big problem.
Bob
Re: immersion oil question
Posted: Sat Sep 07, 2019 5:35 am
by 75RR
One solution would be to not oil the condenser and to lift the cover slip off before dunking the slide.
As it is a well slide, that is where most of the animalcules will be.
Note: Higher magnification objectives have a shallower depth of field, they are not ideal for well slide use.
I lift it off with a pair of tweezers and rinse it in the aquarium, then toss it.
There is no need to toss cover slips after one use when observing pond water. I reuse them several times.
Have a look at Oliver's video. It does look a little stressful at times but it does have a happy ending
Re: immersion oil question
Posted: Sat Sep 07, 2019 1:05 pm
by Roldorf
What do you expect to see from an aquarium with a 100x lens that can't be observed under a 60x? you wouldn't need to use oil at all.
Re: immersion oil question
Posted: Sat Sep 07, 2019 5:04 pm
by Sauerkraut
Perhaps instead of dunking the slide, you could get a squirt bottle that has a good water stream so the microbes could be jetted back into the tank with minimal oil contamination.
Something like this:
https://www.amazon.com/Economy-Bottle-S ... 0497&psc=1
Re: immersion oil question
Posted: Sat Sep 07, 2019 7:14 pm
by mintakax
75RR wrote: ↑Sat Sep 07, 2019 5:35 am
One solution would be to not oil the condenser and to lift the cover slip off before dunking the slide.
As it is a well slide, that is where most of the animalcules will be.
Note: Higher magnification objectives have a shallower depth of field, they are not ideal for well slide use.
I lift it off with a pair of tweezers and rinse it in the aquarium, then toss it.
There is no need to toss cover slips after one use when observing pond water. I reuse them several times.
Have a look at Oliver's video. It does look a little stressful at times but it does have a happy ending
Thanks 75
Yikes on the video ! Resilient creatures they are. I think I'm going to give up on the 100x oil immersion, since I think the depth of field will be driving me nuts anyways.
Re: immersion oil question
Posted: Sat Sep 07, 2019 7:18 pm
by mintakax
Roldorf wrote: ↑Sat Sep 07, 2019 1:05 pm
What do you expect to see from an aquarium with a 100x lens that can't be observed under a 60x? you wouldn't need to use oil at all.
I would certainly prefer 60x. I have10,20,40 and 100x DIC inserts in my turret but not 60x.
Re: immersion oil question
Posted: Sat Sep 07, 2019 7:20 pm
by mintakax
Thanks Heather-- I actually had considered just that.
Re: immersion oil question
Posted: Sat Sep 07, 2019 7:20 pm
by mintakax
wporter wrote: ↑Sat Sep 07, 2019 2:03 am
I would think that the oil, especially if it eventually spreads out to form a sheen or layer, would probably negatively impact the air-water gaseous exchange rate or process.
Completely agree. Thanks.