Stacking Software

Here you can discuss topics such as focus stacking, stitching and other techniques that relate to the processing of micrographs.
Message
Author
MichaelG.
Posts: 1494
Joined: Mon Apr 10, 2017 8:24 am
Location: NorthWest England

Re: Stacking Software

#31 Post by MichaelG. » Sun Dec 23, 2018 9:03 am

janvangastel wrote:A stacking program much used by astrophotographists is autostakkert. It's free and works very well. Maybe worth trying. https://www.autostakkert.com/
Unfortunately, the meaning of the term 'stacking' differs between Astro and Micro work.

In photomicrography, it involves stacking focus [Z-axis] layers to improve the depth-of-field.
In AstroPhotography, it is mostly about alignment in X and Y axes, and noise reduction.

MichaelG.
.

Edit: Here is an good introduction
http://zerenesystems.com/cms/stacker
Too many 'projects'

User avatar
janvangastel
Posts: 132
Joined: Mon Dec 03, 2018 7:05 pm
Location: Huizen, Netherlands
Contact:

Re: Stacking Software

#32 Post by janvangastel » Sun Dec 23, 2018 11:18 am

OK, I didn't know that. Thank you.

User avatar
coominya
Posts: 279
Joined: Sun Mar 11, 2018 11:33 am
Location: Brisbane Aust

Re: Stacking Software

#33 Post by coominya » Tue Jan 22, 2019 1:06 pm

I can use my new camera itself, though I must admit I haven't composed any as yet, nor been near the microscope in weeks :oops: The Olympus M5 camera I bought has consumed my spare time as I have always been a photography buff but haven't had a decent camera in a couple of decades. Catching up for lost time making stop-go movies and shooting wildlife etc.

Anyway this article explains the process inbuilt in the camera. I suppose in a few years everyone may be employing this technology, especially since the speed of the capture is lightening fast and the focus points precisely measured.

https://learnandsupport.getolympus.com/ ... -with-om-d

Quote: The focus stacking feature will shoot 8 photos at the focus positions you set and then merge them into one jpeg file at full resolution. Achieving good results will take some experimenting since so many variables are at play - subject size, distance from subject, lens choice, aperture setting, and focus differential. All eight photos are recorded plus the final stacked image.

Hobbyst46
Posts: 2163
Joined: Mon Aug 21, 2017 9:02 pm

Re: Stacking Software

#34 Post by Hobbyst46 » Tue Jan 22, 2019 1:52 pm

coominya wrote: Quote: The focus stacking feature will shoot 8 photos at the focus positions you set and then merge them into one jpeg file at full resolution. Achieving good results will take some experimenting since so many variables are at play - subject size, distance from subject, lens choice, aperture setting, and focus differential. All eight photos are recorded plus the final stacked image.
From this quote I understand that the camera stacks a set of photos that cover a depth of focus range, and were obtained by automatically spaced focusing with the camera itself. This is not identical to stacking photos created by spaced focusing with the microscope, since the microscope image is optimal when the camera and eyepieces are parfocal. So I am not sure that in-camera automatic shooting-stacking can replace the integration of microscope focusing with shooting, then stacking.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

MicroBob
Posts: 1496
Joined: Sun Dec 25, 2016 9:11 am

Re: Stacking Software

#35 Post by MicroBob » Tue Jan 22, 2019 5:46 pm

Hi Doron,
in theory this is right, but I think that in practice stacking by camera lens focus change will work very well.
It won't be too difficult to compare the results so I would give it a try if I had a camera with stacking function.

Bob

User avatar
coominya
Posts: 279
Joined: Sun Mar 11, 2018 11:33 am
Location: Brisbane Aust

Re: Stacking Software

#36 Post by coominya » Tue Jan 22, 2019 10:49 pm

I didn't realize it when I posted the comment above, but the only way it works is with the camera's auto-focus lens attached. Hence it is probably of limited value in microscopy.

Post Reply