Human Blood smear, Digital Process to Negative effect

Here you can discuss topics such as focus stacking, stitching and other techniques that relate to the processing of micrographs.
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Suphot
Posts: 68
Joined: Wed Mar 11, 2015 4:35 am

Human Blood smear, Digital Process to Negative effect

#1 Post by Suphot » Thu Apr 05, 2018 9:31 am

Human Blood smear, Prepared slide at 400X (40X Objective x 10X Eyepiece)
Afocal Photography using smart phone, zoom in about 2X at smart phone. (Look like 800X Magnification)

Oblique Illumination and Digital Process by using Negative filter effect.
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Human Blood 2 Adj.jpg
Human Blood 2 Adj.jpg (206.3 KiB) Viewed 2640 times

MicroBob
Posts: 1490
Joined: Sun Dec 25, 2016 9:11 am

Re: Human Blood smear, Digital Process to Negative effect

#2 Post by MicroBob » Thu Apr 05, 2018 10:06 am

Great image! How did you prepare the slide?

Here is a blood smear with Pappenheim-stain:
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Blut Pappenheim 40er Apo gestackt groß mit Beschriftung Ausschnitt klein.jpg
Blut Pappenheim 40er Apo gestackt groß mit Beschriftung Ausschnitt klein.jpg (345.94 KiB) Viewed 2635 times

Suphot
Posts: 68
Joined: Wed Mar 11, 2015 4:35 am

Re: Human Blood smear, Digital Process to Negative effect

#3 Post by Suphot » Thu Apr 05, 2018 1:27 pm

Hi MicroBob,

I did not prepare the slide by myself. I bought this prepared slides set from ebay. Your slide is much clearer and contain more details, very beautiful one.



Suphot

MicroBob
Posts: 1490
Joined: Sun Dec 25, 2016 9:11 am

Re: Human Blood smear, Digital Process to Negative effect

#4 Post by MicroBob » Thu Apr 05, 2018 3:13 pm

I actually like your inverted image very much. Is is very contrasty and colourful.
I have inverted your image back - it might have been stained the same way. I think that Pappenheim stain is a widely used blood stain.
My slide was made at a meeting of our microscopy group under supervision of a very knowledgable person. As always in microscopy you need a couple of substances you don't already have in your cupboard. :roll:

Bob
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Blut1.jpg
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Suphot
Posts: 68
Joined: Wed Mar 11, 2015 4:35 am

Re: Human Blood smear, Digital Process to Negative effect

#5 Post by Suphot » Fri Apr 06, 2018 3:01 am

Hi MicroBob,

Thank you very much for your comment and for post the original photo of Human Blood smear.

It is very good to see it in Negative filter. Sometime it look like plant cell when the color is green.


Cheers,


Suphot

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