MicrobeHunter.com Microscopy Forum

Human Blood smear, Prepared slide at 400X (40X Objective x 10X Eyepiece)
Afocal Photography using smart phone, zoom in about 2X at smart phone. (Look like 800X Magnification)

Oblique Illumination and Digital Process by using Negative filter effect.

Great image! How did you prepare the slide?

Here is a blood smear with Pappenheim-stain:

Hi MicroBob,

I did not prepare the slide by myself. I bought this prepared slides set from ebay. Your slide is much clearer and contain more details, very beautiful one.



Suphot

I actually like your inverted image very much. Is is very contrasty and colourful.
I have inverted your image back - it might have been stained the same way. I think that Pappenheim stain is a widely used blood stain.
My slide was made at a meeting of our microscopy group under supervision of a very knowledgable person. As always in microscopy you need a couple of substances you don't already have in your cupboard.

Bob

Hi MicroBob,

Thank you very much for your comment and for post the original photo of Human Blood smear.

It is very good to see it in Negative filter. Sometime it look like plant cell when the color is green.


Cheers,


Suphot