Gradient light interference microscopy
Gradient light interference microscopy
Here's a paper for those interested in this new technique:
https://www.nature.com/articles/s41467-017-00190-7
A little beyond us kitchen-sink microscopists, but interesting.
from the introduction: "...imaging optically thick, multiple scattering specimens is still challenging for any optical method, including QPI. The fundamental obstacle is that multiple scattering generates an incoherent background, which ultimately degrades the image contrast. An imaging method dedicated to imaging these thick specimens must include a mechanism to subdue the multiple scattering backgrounds and exhibit strong sectioning to suppress the out-of-focus light. To overcome these challenges, here we introduce a new QPI method, referred to as gradient light interference microscopy (GLIM). GLIM combines DIC microscopy with low-coherence interferometry and holography."
https://www.nature.com/articles/s41467-017-00190-7
A little beyond us kitchen-sink microscopists, but interesting.
from the introduction: "...imaging optically thick, multiple scattering specimens is still challenging for any optical method, including QPI. The fundamental obstacle is that multiple scattering generates an incoherent background, which ultimately degrades the image contrast. An imaging method dedicated to imaging these thick specimens must include a mechanism to subdue the multiple scattering backgrounds and exhibit strong sectioning to suppress the out-of-focus light. To overcome these challenges, here we introduce a new QPI method, referred to as gradient light interference microscopy (GLIM). GLIM combines DIC microscopy with low-coherence interferometry and holography."
Re: Gradient light interference microscopy
Bill, about a year and a half ago 75RR posted a site;
http://www.photomacrography.net/forum/v ... hp?t=15142
that allows us kitchen-sink microscopists to simulate the effect.
Thanks for the article. It made my head hurt
http://www.photomacrography.net/forum/v ... hp?t=15142
that allows us kitchen-sink microscopists to simulate the effect.
Thanks for the article. It made my head hurt
Re: Gradient light interference microscopy
JimT,
I am guessing you were referring to this thread (your original link does not work):
http://www.photomacrography.net/forum/v ... hp?t=15142
I am guessing you were referring to this thread (your original link does not work):
http://www.photomacrography.net/forum/v ... hp?t=15142
Re: Gradient light interference microscopy
Yes. Thanks for the edit. As I said, we kitchen sink microscopists need all the help we can get.
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Re: Gradient light interference microscopy
after reading that link, I clearly realized that GLIM is no doubt a radical improvement over the DLIM microscopy that I have been practicing, it seems also for about 4 centuries. DLIM is an acronym for Dim, Limited, Interfered with Microscopy.wporter wrote:Here's a paper for those interested in this new technique:
https://www.nature.com/articles/s41467-017-00190-7
A little beyond us kitchen-sink microscopists, but interesting.
from the introduction: "...imaging optically thick, multiple scattering specimens is still challenging for any optical method, including QPI. The fundamental obstacle is that multiple scattering generates an incoherent background, which ultimately degrades the image contrast. An imaging method dedicated to imaging these thick specimens must include a mechanism to subdue the multiple scattering backgrounds and exhibit strong sectioning to suppress the out-of-focus light. To overcome these challenges, here we introduce a new QPI method, referred to as gradient light interference microscopy (GLIM). GLIM combines DIC microscopy with low-coherence interferometry and holography."
Re: Gradient light interference microscopy
That sounds similar to ISM-FTS (I See More Floaters Than Subjects) microscopy.
Re: Gradient light interference microscopy
The high-flown technical concepts under discussion are interesting and all, but for my money "kitchen-sink microscopists" is one of the finest contributions to this forum ever. Good on you sir, and a toast your way with words!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: Gradient light interference microscopy
Must agree with you Kurt. Why only this morning my Wife thought I'd cut my arm off when all I'd been doing (at said sink) was rinsing an empty basic-Fuchsin bottle!KurtM wrote:The high-flown technical concepts under discussion are interesting and all, but for my money "kitchen-sink microscopists" is one of the finest contributions to this forum ever. Good on you sir, and a toast your way with words!
John B
Re: Gradient light interference microscopy
I completely agree to posting the quick version of any subject.. This makes it much easier for all of us to get up and running with a new idea, but some of us sometimes like to know the "how" and "why" of something..KurtM wrote:The high-flown technical concepts under discussion are interesting and all, but for my money "kitchen-sink microscopists" is one of the finest contributions to this forum ever. Good on you sir, and a toast your way with words!
BillT