Rheinberg illumination at higher powers

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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GeekyWife
Posts: 27
Joined: Wed Nov 18, 2020 9:19 pm

Rheinberg illumination at higher powers

#1 Post by GeekyWife » Thu Jan 21, 2021 1:20 pm

I recently printed some Rheinberg filters in various color combinations using a color printer and transparency material. Complementary colors such as yellow/violet and orange/blue work very well. I'm very pleased with the results.

However, I can only get it to work well with a 4X or 10X objective. That limits me to a maximum of 200X, using the 10X objective and a 20X eyepiece.

If I switch to a 20X objective, the background no longer appears to be the color of the center of the filter, and the effect is lost.

Has anyone had success with Rheinberg illumination using a 20X or 40X objective? Is it just a matter of making the center spot larger, or is there more to it than that?

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mrsonchus
Posts: 4175
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Rheinberg illumination at higher powers

#2 Post by mrsonchus » Thu Jan 21, 2021 2:04 pm

Hi, it's a lot like darkfield patches - higher magnifications need larger center spots for the background.
John B

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