First session with Fluorescence

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
Post Reply
Message
Author
macnmotion
Posts: 549
Joined: Mon Sep 26, 2022 3:13 am

First session with Fluorescence

#1 Post by macnmotion » Sun Oct 29, 2023 3:35 pm

Today I got the fluorescence system working with my Nikon inverted diaphot. Here are a couple of short video clips:

First, pondwater stained with Acridine Orange, which emits green light through a FITC cube:


Second, pondwater autofluorescence using a red emission TRIITC cube:


Here are a couple of stills. This is proof of concept, there's plenty I have to learn.
FITC-Acridine-Orange.jpg
FITC-Acridine-Orange.jpg (25.87 KiB) Viewed 949 times
fluorescence-first-autofluorescence-20x-plan-apo_sm.jpg
fluorescence-first-autofluorescence-20x-plan-apo_sm.jpg (91.4 KiB) Viewed 949 times
Top
Post Reply

Return to “Illumination Techniques”