MicrobeHunter.com Microscopy Forum

You can also access this page with: www.microscopy-forum.com
It is currently Thu Aug 22, 2019 5:50 pm

All times are UTC

Post new topic  Reply to topic  [ 3 posts ] 
Author Message
PostPosted: Sat Mar 19, 2016 5:48 am 

Joined: Sat Mar 19, 2016 5:38 am
Posts: 1
Dear all,
I believe that all of you are experts in microscopy field. Now I bought a Confocal Microscopy from my colleague to observe the whole process of experiment on biotin peptide. I am wondering if this is proper?

PostPosted: Sun Mar 20, 2016 6:22 pm 

Joined: Fri May 15, 2015 12:15 am
Posts: 2641
Rather than conventional methods of biochemical assay? Probably too much microscope. They are very sensitive to tiny imperfections in coverglass quality or contamination in the optical path too, so require extreme precision and care in use. Wide field fluorescent microscopes are more forgiving and provide the information anyway. I don't use my fluorescent microscope much, so we should trade.

PostPosted: Wed Mar 30, 2016 2:15 pm 

Joined: Wed Jul 29, 2015 3:18 am
Posts: 66
What exactly is it you're trying to figure out?

The confocal is also capable of epifluorescence, at least the one I use also has an LED source for 405, 488, 561, 640. That link goes to vasopression with BIotin tagged to it, are you going to use an anti-biotin antibody and then secondaries to see where the vasopressin is after you've added it to something?

Also I didn't think a confocal fluorescence setup is something you just buy go off and buy, are you part of lab or PI or something?

Display posts from previous:  Sort by  
Post new topic  Reply to topic  [ 3 posts ] 

All times are UTC

Who is online

Users browsing this forum: No registered users and 3 guests

You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
Jump to:  
Powered by phpBB® Forum Software © phpBB Limited