My silly quest for : 'poor mans DIC'.

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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charlie g
Posts: 1849
Joined: Mon Oct 20, 2014 7:54 pm

My silly quest for : 'poor mans DIC'.

#1 Post by charlie g » Sun Apr 08, 2018 7:28 pm

Recently, coominya and 75RR posted into a thread: "The essence of DIC" with great links. In these links,and other microscopy portals...it encouraged me to set up a pol contrast/Kholer illumination light path...and then to add the substage, and the objective lens Normarski prisms.

My mission is to play the variety of orientations of the two pol-filters, and then try a variety of substage prism orientations with respect to the objective prism.

A totally separate issue is my 'lousy' image capture method with the trinoc vertical port of the Olympus stand. I need better control of the illumination...of course visually the test subjects look better than my image captures!

Radiolaria skeletons are so huge, compared to diatoms as a test subject for the 40X objective I am using for this 'attempt to develop DIC contrast'. This project needs a lot more attention before I post to forum images and questions, again, thank you all kind and sharing DIC forum microscopists. Charlie guevara
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charlie g
Posts: 1849
Joined: Mon Oct 20, 2014 7:54 pm

Re: My silly quest for : 'poor mans DIC'.

#2 Post by charlie g » Sun Apr 08, 2018 7:37 pm

So I need to try a variety of substage prism orientations...perhaps use diatom frustules, rather than these huge Radiolaria skeletons. Thanks for your patience..and please opine on the prism orientations, and the pol-filter orientations. Charlie Guevara..I need to purchase a DIC microscope when the seller upgrades to a better stand.
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billbillt
Posts: 2895
Joined: Mon Nov 10, 2014 10:01 pm

Re: My silly quest for : 'poor mans DIC'.

#3 Post by billbillt » Sun Apr 08, 2018 9:09 pm

Hi Charlie,

I don't see this as silly at all.. You have a great potential in achieving your desired results with what you posted here.. Please keep us informed and good luck...

The Best,
BillT

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coominya
Posts: 279
Joined: Sun Mar 11, 2018 11:33 am
Location: Brisbane Aust

Re: My silly quest for : 'poor mans DIC'.

#4 Post by coominya » Mon Apr 09, 2018 7:20 am

Well beyond me, I'm just a black box operator at this stage but keep us posted on what comes out of the EP. I found a DIC kit for my olympus imt-2 going for a grand and a half (they are generally 6 grand) but it was an Indonesian company that accepts only wire transfers or cash, no CC or paypal so I figured it's a scam. Their website has only been up for 6 months as well, a construction company.

Your olympus looks a lot like mine, an old ECE from the late 1960's. I love the quality of it, everything is so well machined compared to modern microscopes I have looked at in the student range. So glad I bought it.

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