Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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While experimenting with my ongoing EPI journey I inadvertently took this image. I will not disclose what it is of, but maybe others have seen similar before. It was not taken with an EPI objective, just a standard APO one (10x) using vertical illumination. Photographed with a Nikon D300s fitted to a Nikon S series microscope. The APO is a Lomo one. It is, of course, a single image, i.e., no stacking. Note: the second higher contrast image is using a standard non-APO Nikon objective.
- 2019-05-30 20-48-58 (B,Radius8,Smoothing4).jpg (146.23 KiB) Viewed 2924 times
- Mystery !.jpg (240.4 KiB) Viewed 2969 times
- Mystery 2.jpg (156.69 KiB) Viewed 2972 times