LED vs. Halogen test

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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Radazz
Posts: 982
Joined: Sat Jun 18, 2016 11:55 am
Location: Arnold, Missouri USA

LED vs. Halogen test

#1 Post by Radazz » Mon Jan 06, 2020 9:58 pm

Here’s a direct comparison.
Zeiss Standard
MD500 5mp eyepiece camera ToupView (some frequency problems)

Illumination
LED - RetroDiode Zeiss Standard replacement illuminator
Halogen - Wild Heerbrugg Hal lamp with transformer

Both fit into a tunnel on the back of the base.

100x
Antique slide Fossil Diatoms Circle Group Wolfish Bay

Image
Diatoms Wolfish Bay LED

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Diatoms Wolfish Bay Halogen

Here’s one from Mrsonchus!
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Mitosis root tip LED

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Mitosis root tip Halogen

35x
Acetaminophen Polarized illumination
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Acetaminophen LED

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Acetaminophen Halogen

Perhaps I should have run such a test before opening my mouth.

Enjoy,
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40

Hobbyst46
Posts: 2948
Joined: Mon Aug 21, 2017 9:02 pm

Re: LED vs. Halogen test

#2 Post by Hobbyst46 » Mon Jan 06, 2020 10:21 pm

Radazz, I like very much this comparison ! thanks for posting !
Was the LED white (6500K) ? was the LED light filtered ?
On my microscope, equipped with a retroDiode white LED, I use a KR12 warming filter to reduce the bluish-violet tint somewhat.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

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Radazz
Posts: 982
Joined: Sat Jun 18, 2016 11:55 am
Location: Arnold, Missouri USA

Re: LED vs. Halogen test

#3 Post by Radazz » Mon Jan 06, 2020 11:09 pm

Hobbyst46 wrote:
Mon Jan 06, 2020 10:21 pm
Radazz, I like very much this comparison ! thanks for posting !
Was the LED white (6500K) ? was the LED light filtered ?
On my microscope, equipped with a retroDiode white LED, I use a KR12 warming filter to reduce the bluish-violet tint somewhat.
6500k yes.the first one.
Don’t have such a filter.

Also the two microscopes I use for photography are infinity, Olympus IX70 and BX40, both halogen,which sort of widens the gap.

Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40

david_b
Posts: 150
Joined: Wed Aug 28, 2019 1:07 pm

Re: LED vs. Halogen test

#4 Post by david_b » Mon Jan 06, 2020 11:50 pm

Very good, thank you!
The first LED image shows quite a lot of CA compared to the halogen. Is that banding on the slide?
And it's interesting how differently the colours are rendered in the last pair.

rs6000
Posts: 92
Joined: Fri Jul 13, 2018 4:14 pm

Re: LED vs. Halogen test

#5 Post by rs6000 » Wed Jan 08, 2020 4:28 pm

Hello do you mind if I ask you more specifics on what your setup for the polarized image you posted taken from a Zeiss STd my zeiss does not have the pol knob function installed on the nosepiece limb, I have to hold a polarizer above the eyepiece to get the effect
I have not seen many with that option in the head but old catalogs do show this as available Ive just never seen on for sale.

cheers

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Radazz
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Joined: Sat Jun 18, 2016 11:55 am
Location: Arnold, Missouri USA

Re: LED vs. Halogen test

#6 Post by Radazz » Thu Jan 09, 2020 2:58 pm

I bought a retro fit set from an auction site. Polarizer sized to sit on the field lens, and an analyzer that drops into the nose piece above the objectives.
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40

rs6000
Posts: 92
Joined: Fri Jul 13, 2018 4:14 pm

Re: LED vs. Halogen test

#7 Post by rs6000 » Thu Jan 09, 2020 5:17 pm

That's a cool Idea Very nice
I thought about doing that but no filter I could find was small enough to fit as there is not much room inside the head
the method of holding one of the polarizers between your eye and the eyepiece is pretty precarious esp if you want to take pictures

david_b
Posts: 150
Joined: Wed Aug 28, 2019 1:07 pm

Re: LED vs. Halogen test

#8 Post by david_b » Fri Jan 10, 2020 5:33 pm

Here's my offering...

Image

Image

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