Dark Field Microscopy Trainers

What is your microscopy history? What are your interests? What equipment do you use?
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apochronaut
Posts: 6327
Joined: Fri May 15, 2015 12:15 am

Re: Dark Field Microscopy Trainers

#1 Post by apochronaut » Fri Nov 24, 2017 2:42 pm

What this all boils down to is that being able to view a live blood sample in DF, does not mean that you know what you are looking at. Blood is very complex.

I had some contact many years ago with one of the earlier practitioners of this now quite popular practice. He was a hematologist and using a very high quality instrument( a BX 40, with planapochromats), DF and fluorescence, he was able to make a lot of quite rational assumptions about a patient's condition. He had long experience and had been able to match patient after patient with what their blood revealed. The popularization of the practice, now, means that with a several day course and a shiny new Chinese achromat microscope, hundreds of people are able to set up shop providing a service that actually takes experience, to know how to practice properly. Some, will however gain experience. It is not the technique that is at fault, it is the lack of proper accreditation.

Modern medicine is constantly improving but at the same time has a tendency to drop older techniques that are costly or unreliable due to practitoner error. Practitioner error, not technique error. Looking at the blood, is now done almost entirely in licensed labs and the number of techniques employed is carefully regulated. Insurance companies, increasingly run the system. Doctors used to do DF live blood analysis in their office, to test for syphilis. They don't do that any more, it's done in a lab but the disease is still under diagnosed because usually, what is being looked for is the presence of spirochaetes. The missing piece of the syphilis puzzle, is that the organism is pleomorphic, a well recognized phenomena of microorganisms, whereby they change their form due to various factors. You can have syphilis and not present with a spirochaete. You can have Lyme disease and not present with a spirochaete. The very sad thing is, that our conventional western medicine fraternity barely knows of the existence of pleomorphism. In general, they still think that a bacteria is a bacteria is a bacteria and if it is a coccus, it will be small and round and if it is a bacillus it will be rod shaped and on and on. They are as a group, blissfully ignorant of the threat that pleomorphic and cell wall deficient forms of microorganisms pose to the populace. At the very least, that is one thing that live blood analysis aficionados are looking for.
Borrellia Burgdorferii, the causative agent of Lyme disease, according to some researchers, Eva Sapi, working at the U. of Hew Haven, https://www.youtube.com/watch?v=AmvgOfIN_8c and Ying Zhang at Johns Hopkins https://on-lyme.org/en/sufferers/lyme-s ... ronic-lyme has a very complex pleomorphism and looking for evidence of it's existence in live blood with DF is a primary starting point for a diagnosis. Right now, with ELISA and Western Blot being the primary tools, diagnosis is pathetic...but it is cheap, and an idiot can run the tests. It takes a skilled , experienced practioner to do DF , not to mention DF/fluorescence.

For more on the pioneers of pleomorphism here are some wikipedia entries for 2 of them. One, Lida Mattman was nominated for a nobel prize.
https://en.wikipedia.org/wiki/Lida_Holmes_Mattman
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC380327/

Gerald Domingue
https://en.wikipedia.org/wiki/Gerald_Domingue
http://bacteriality.com/2007/08/domingue/
.... just a couple of people who understood the significance of pleomorphism to human health. It would be nice if the medical fraternity would catch up.

ajmckay
Posts: 21
Joined: Sat Dec 09, 2017 5:39 pm

Re: Dark Field Microscopy Trainers

#2 Post by ajmckay » Tue Dec 12, 2017 4:08 pm

Nice read - thanks for taking the time to write. In searching the term "darkfield" recently it seems like a lot of the results are geared toward live blood analysis services or sites that want you to buy related materials...

apochronaut
Posts: 6327
Joined: Fri May 15, 2015 12:15 am

Re: Dark Field Microscopy Trainers

#3 Post by apochronaut » Tue Dec 12, 2017 6:29 pm

Thank you and just a note, to explain this thread. It was originally an advertising pitch for a DF Training course, out of the UK. That was followed by a number of negative comments. The original pitch has been deleted, along with bulk of the comments, so my post is in fact a response, not an original post.
There is a saying " only a fool tests the depth of the water with both feet" and that holds true, for many who have placed themselves on opposite sides of the L.B.A. river.

ajmckay
Posts: 21
Joined: Sat Dec 09, 2017 5:39 pm

Re: Dark Field Microscopy Trainers

#4 Post by ajmckay » Thu Dec 21, 2017 7:16 am

Not sure what the L.B.A. river is lol.

Given your further explanation of the post I suppose I'm a little confused at why a DF training course would receive negative comments? Obviously what's been written has already passed so it needn't be dredged up again - more of a rhetorical I suppose!

My prior post was a weak attempt to say that I find a lack of any substantiable information on DF application. There are some sources that give some decent tips on technique but that's only helpful to a point. As a hobbyist the allure of DF is in the aesthetics (for me). I'm not going to be doing any real analysis myself in all honesty. So a training course that teaches that application probably wouldn't interest me. But that being said I still wish there were a bit more information available on a more casual basis as I'm sure there are a lot of great applications for DF that I haven't even thought of.

MichaelG.
Posts: 4026
Joined: Mon Apr 10, 2017 8:24 am
Location: North Wales

Re: Dark Field Microscopy Trainers

#5 Post by MichaelG. » Thu Dec 21, 2017 8:47 am

apochronaut wrote:Thank you and just a note, to explain this thread. It was originally an advertising pitch for a DF Training course, out of the UK. That was followed by a number of negative comments. The original pitch has been deleted, along with bulk of the comments, so my post is in fact a response, not an original post.
Thank you for that note of explanation ^^^

For ajmcay's benefit, I am re-posting this link:
http://www.mold-survivor.com/a_modern_s ... ctive.html
.. it seems a well-balanced discussion of Enderlein's 'concept' and I hope the forum moderators will not object.

MichaelG.
Too many 'projects'

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