Like the $995 trinocular Zeiss scope with DIC that 75RR linked to...
New here! Questions about the Big Four
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Re: New here! Questions about the Big Four
Re: New here! Questions about the Big Four
Re: New here! Questions about the Big Four
Thanks, already gone, unfortunately!75RR wrote: ↑Thu Aug 13, 2020 6:52 pmHere is an interesting one: https://www.ebay.com/itm/Carl-Zeiss-Mic ... Swx4hc8sAl
Got some nice bits on it!
Re: New here! Questions about the Big Four
Hey Pete,PeteM wrote: ↑Thu Aug 13, 2020 7:39 pmBacteria are tiny and hard to see even at 400x. About all you get is the rough outline (rod shaped, etc.) - which may be enough? Darkfield illumination and 100x can both help get a somewhat better ID (DIC, too, but $$$). Which suggests you want a scope with fairly bright illumination.Geode wrote: ↑Thu Aug 13, 2020 6:36 pm. . .
My professor says phase contrast isn't ideal for our application (at least for the method she is describing, where we do Kohler illumination and then close down the iris at the end to bring up the contrast and be able to count all the bacteria at 400x - what she is calling "shadowing").
I am looking to add phase contrast for my own experimentation and flexibility as my needs expand over the next several years.
For the reports/counts I'll be doing, the shape is pretty much sufficient. My course doesn't involve IDing bacteria much further than the general morphological types.