New here! Questions about the Big Four

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viktor j nilsson
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Location: Lund, Sweden

Re: New here! Questions about the Big Four

#31 Post by viktor j nilsson » Fri Aug 14, 2020 4:06 am

Zuul wrote:
Thu Aug 13, 2020 11:27 pm
Geode wrote:
Thu Aug 13, 2020 6:38 pm
Thanks, I had filtered for trinocular scopes.. so that one didn't show up.
I'm still searching, so there's a lot to filter through :)
Many microscopes on ebay that have a trinocular head are not listed that way, so be careful with your search and filtering. Remember that frequently the best deals are to be had when the description is lacking or flat out wrong.
Like the $995 trinocular Zeiss scope with DIC that 75RR linked to...

Geode
Posts: 42
Joined: Wed Aug 05, 2020 1:13 pm

Re: New here! Questions about the Big Four

#32 Post by Geode » Sat Aug 15, 2020 6:15 pm

Zuul wrote:
Thu Aug 13, 2020 11:27 pm
Geode wrote:
Thu Aug 13, 2020 6:38 pm
Thanks, I had filtered for trinocular scopes.. so that one didn't show up.
I'm still searching, so there's a lot to filter through :)
Many microscopes on ebay that have a trinocular head are not listed that way, so be careful with your search and filtering. Remember that frequently the best deals are to be had when the description is lacking or flat out wrong.
Thanks, will keep looking!

Geode
Posts: 42
Joined: Wed Aug 05, 2020 1:13 pm

Re: New here! Questions about the Big Four

#33 Post by Geode » Sat Aug 15, 2020 6:16 pm

75RR wrote:
Thu Aug 13, 2020 6:52 pm
Here is an interesting one: https://www.ebay.com/itm/Carl-Zeiss-Mic ... Swx4hc8sAl

Got some nice bits on it!
Thanks, already gone, unfortunately!

Geode
Posts: 42
Joined: Wed Aug 05, 2020 1:13 pm

Re: New here! Questions about the Big Four

#34 Post by Geode » Sat Aug 15, 2020 6:17 pm

PeteM wrote:
Thu Aug 13, 2020 7:39 pm
Geode wrote:
Thu Aug 13, 2020 6:36 pm
. . .

My professor says phase contrast isn't ideal for our application (at least for the method she is describing, where we do Kohler illumination and then close down the iris at the end to bring up the contrast and be able to count all the bacteria at 400x - what she is calling "shadowing").
I am looking to add phase contrast for my own experimentation and flexibility as my needs expand over the next several years.
Bacteria are tiny and hard to see even at 400x. About all you get is the rough outline (rod shaped, etc.) - which may be enough? Darkfield illumination and 100x can both help get a somewhat better ID (DIC, too, but $$$). Which suggests you want a scope with fairly bright illumination.
Hey Pete,
For the reports/counts I'll be doing, the shape is pretty much sufficient. My course doesn't involve IDing bacteria much further than the general morphological types.

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