'Test' slide - Part 1

Everything relating to microscopy hardware: Objectives, eyepieces, lamps and more.
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TonyT
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Joined: Fri Sep 11, 2020 8:30 pm

'Test' slide - Part 1

#1 Post by TonyT » Sun Sep 13, 2020 7:57 pm

A mounted (Canada Balsam mountant, cover slip) mosquito wing makes a readily available (mosquitoes can be found everywhere, in season) subject for testing the resolution quality of a microscope setup.

I plan to use this wing with the following setup:
Olympus BH2/BHS
NFK 2.5x projection eyepiece
flash instead of the standard 100w bulb
Nikon D810, full-frame DSLR
4x, 10x, 20x, 40x objectives.

First test with the Oly 4x D Plan
top image: full frame, 7360x4912 pixels, reduced to 1024 px wide (single image)
2nd image: 7 images stacked with Zerene Stacker, cropped and reduced to 1024 px wide
3rd image: 1024 actual pixels, crop of the 6,300 px that constitute the entire wing length.

The 4x gives a good image for the entire wing; not realistic to use a 4x to image just the wing tip.
Attachments
13ix20 wing 1.jpg
13ix20 wing 1.jpg (89.27 KiB) Viewed 427 times
13ix20 wing 2.jpg
13ix20 wing 2.jpg (130.46 KiB) Viewed 427 times
13ix20 wing 3.jpg
13ix20 wing 3.jpg (246.23 KiB) Viewed 427 times

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