'Test' slide - Part 1
Posted: Sun Sep 13, 2020 7:57 pm
A mounted (Canada Balsam mountant, cover slip) mosquito wing makes a readily available (mosquitoes can be found everywhere, in season) subject for testing the resolution quality of a microscope setup.
I plan to use this wing with the following setup:
Olympus BH2/BHS
NFK 2.5x projection eyepiece
flash instead of the standard 100w bulb
Nikon D810, full-frame DSLR
4x, 10x, 20x, 40x objectives.
First test with the Oly 4x D Plan
top image: full frame, 7360x4912 pixels, reduced to 1024 px wide (single image)
2nd image: 7 images stacked with Zerene Stacker, cropped and reduced to 1024 px wide
3rd image: 1024 actual pixels, crop of the 6,300 px that constitute the entire wing length.
The 4x gives a good image for the entire wing; not realistic to use a 4x to image just the wing tip.
I plan to use this wing with the following setup:
Olympus BH2/BHS
NFK 2.5x projection eyepiece
flash instead of the standard 100w bulb
Nikon D810, full-frame DSLR
4x, 10x, 20x, 40x objectives.
First test with the Oly 4x D Plan
top image: full frame, 7360x4912 pixels, reduced to 1024 px wide (single image)
2nd image: 7 images stacked with Zerene Stacker, cropped and reduced to 1024 px wide
3rd image: 1024 actual pixels, crop of the 6,300 px that constitute the entire wing length.
The 4x gives a good image for the entire wing; not realistic to use a 4x to image just the wing tip.