LWD objective on 0.18 cover slip slide.
LWD objective on 0.18 cover slip slide.
Hi,
I just started and made a silly problem. Instead of buying a finite 20x 160/0.17 I bought a finite LPL LWD 25x 160/1.5. What is the compromise of using this instead of the one for 0.17 cover slip? My intention is using it to capture crystal on slide, watch some dyed slide or slide without cover slip.
I just started and made a silly problem. Instead of buying a finite 20x 160/0.17 I bought a finite LPL LWD 25x 160/1.5. What is the compromise of using this instead of the one for 0.17 cover slip? My intention is using it to capture crystal on slide, watch some dyed slide or slide without cover slip.
Re: LWD objective on 0.18 cover slip slide.
Easily-done! I suspect that one's for inverted microscopy - hence the LWD and the thick cover-slip (petri dish bottom)...
Personally I'd re-list that one and crack on with looking for the correct one - you'll only ever be compromising with this one, better to start with the right one/s. Just a case of experience old chap, I've lost count of the mistakes I've made as I've developed my microscopy, as have we all I think.
Personally I'd re-list that one and crack on with looking for the correct one - you'll only ever be compromising with this one, better to start with the right one/s. Just a case of experience old chap, I've lost count of the mistakes I've made as I've developed my microscopy, as have we all I think.
John B
Re: LWD objective on 0.18 cover slip slide.
Hi,
in theory the 0,17mm instead of 1,5mm glass layer will introduce abberations to some extent. But as you already have it I would suggest to just compare it with the weaker and stronger objectives you have an see how well it performs. I would also look for parfocality and especially check for image quality in the plane of sharpness and above and below. You can then compare to the image of the slide with cover slip down and see whether you find much difference. The objective might work well. Zeiss 25:1 160mm objectives are not as common as 10:1 and 40:1 so you might have to look for one for a while.
Bob
in theory the 0,17mm instead of 1,5mm glass layer will introduce abberations to some extent. But as you already have it I would suggest to just compare it with the weaker and stronger objectives you have an see how well it performs. I would also look for parfocality and especially check for image quality in the plane of sharpness and above and below. You can then compare to the image of the slide with cover slip down and see whether you find much difference. The objective might work well. Zeiss 25:1 160mm objectives are not as common as 10:1 and 40:1 so you might have to look for one for a while.
Bob
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Re: LWD objective on 0.18 cover slip slide.
so have you actually tried it to see how good/bad the image is?Kionka wrote: ↑Sat Nov 28, 2020 9:05 amHi,
I just started and made a silly problem. Instead of buying a finite 20x 160/0.17 I bought a finite LPL LWD 25x 160/1.5. What is the compromise of using this instead of the one for 0.17 cover slip? My intention is using it to capture crystal on slide, watch some dyed slide or slide without cover slip.
Do so, then put the slide upside down on your stage, and things should improve markedly.
I realise this is a faff if you were to do it each time, and would require adjustment from your other lenses, but it might let you see if the performance is so bad it isn't useable.
Re: LWD objective on 0.18 cover slip slide.
Thanks a lot, I will get the correct 20x or 25x and put this on sale.
I still have a question. I got an eyepiece thanks to mrsonchus. It is parfocal when I look through the photo eyepiece and the binocular, however when I put my camera with the adapter on it, it will be out of focus and the edges are extremely unsharp.
Through eyepiece
Through photo eyepiece
Camera through photo eyepiece without 13mm to sensor stacking
Camera through photo eyepiece with 13mm to sensor stacking
Setup with stacking.
I use a K mount macro tube connects to k mount - e mount adapter.
I still have a question. I got an eyepiece thanks to mrsonchus. It is parfocal when I look through the photo eyepiece and the binocular, however when I put my camera with the adapter on it, it will be out of focus and the edges are extremely unsharp.
Through eyepiece
Through photo eyepiece
Camera through photo eyepiece without 13mm to sensor stacking
Camera through photo eyepiece with 13mm to sensor stacking
Setup with stacking.
I use a K mount macro tube connects to k mount - e mount adapter.
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Re: LWD objective on 0.18 cover slip slide.
Do you have a lens between the camera and eyepiece? Most Zeiss setups of this era would have used one.
Re: LWD objective on 0.18 cover slip slide.
If the eyepice is parfocal when sitting in the photo port it won't project an image on you sensor up there. When you lift it up ca. 10mm you might get parfocality.
Bob
Bob
Re: LWD objective on 0.18 cover slip slide.
I raised it about 5mm and got these, look better but I think I expected more from it.
with 10x objective
with 2.5x objective
I use a meiji 2.5x photo eyepiece.Scarodactyl wrote: ↑Sat Nov 28, 2020 5:22 pmDo you have a lens between the camera and eyepiece? Most Zeiss setups of this era would have used one.
Re: LWD objective on 0.18 cover slip slide.
Is that made with a 2,5:1 objective? And is it a plan objective?
Re: LWD objective on 0.18 cover slip slide.
The Plan 2,5 is quite a nice objective, see here: https://www.mikroskopie-forum.de/index. ... ic=39220.0
The image in the link is better than I would have thought possible with a single shot and this objective. But this doesn't happen by itself. The usual condensers are not really optimized for this low magnification and big object area. You might get a better image when lowering the condenser or putting a paper tissue or other diffuasor on top of the condenser top lens. You might also remove the top lens or the complete condenser. Then a thin section is better for use with these low power objectives.
The image in the link is better than I would have thought possible with a single shot and this objective. But this doesn't happen by itself. The usual condensers are not really optimized for this low magnification and big object area. You might get a better image when lowering the condenser or putting a paper tissue or other diffuasor on top of the condenser top lens. You might also remove the top lens or the complete condenser. Then a thin section is better for use with these low power objectives.
Re: LWD objective on 0.18 cover slip slide.
My condenser has a flip top and according to the manual it can be used for 2.5x.MicroBob wrote: ↑Sun Nov 29, 2020 8:03 pmThe Plan 2,5 is quite a nice objective, see here: https://www.mikroskopie-forum.de/index. ... ic=39220.0
The image in the link is better than I would have thought possible with a single shot and this objective. But this doesn't happen by itself. The usual condensers are not really optimized for this low magnification and big object area. You might get a better image when lowering the condenser or putting a paper tissue or other diffuasor on top of the condenser top lens. You might also remove the top lens or the complete condenser. Then a thin section is better for use with these low power objectives.
I just tried to compare the 2.5x with no condenser and with it.
Without the condenser
With the condenser and no top element.
The funny thing is when looking at the binocular, the condenser gives better image than without it.
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Re: LWD objective on 0.18 cover slip slide.
Oh, there's your problem. I don't know what compensation meiji uses in their scopes, but Zeiss used about the strongest compensations of their era. The correct Zeiss eyepiece is vital to getting good photos since they are delicately matched with thr objectives to make a complete optical system.
Re: LWD objective on 0.18 cover slip slide.
What puzzles me: Why do the images have a black border? To me this looks as if the section is nat fully illuminated.
Bob
Bob
Re: LWD objective on 0.18 cover slip slide.
Or maybe I am too picky on this.Scarodactyl wrote: ↑Sun Nov 29, 2020 10:33 pmOh, there's your problem. I don't know what compensation meiji uses in their scopes, but Zeiss used about the strongest compensations of their era. The correct Zeiss eyepiece is vital to getting good photos since they are delicately matched with thr objectives to make a complete optical system.
The black borders are because of cross polarization Bob.