MicrobeHunter.com Microscopy Forum

Last night I found some beautiful needle shaped (with flared ends) diatoms in a new pond sample, decided to do a comparison of the LOMO 40x Plan Apo objective with K10x/18 eyepieces on the LOMO L-2-2 stand against the Olympus BH2 with 40x DPLAN objective.
The Olympus setup gave a nice view but with a fair bit of false color around the shell and in the oil droplets inside the diatom.
The LOMO setup gave a nice view with practically no false color in the same areas. Same slide, same diatom. I was very pleased with this result. This was bright field illumination, I did not attempt oblique or anything else.
It might not be as good as it could be if I had all the properly matched parts, but I'm going to call it good, at least for now. If I can get any decent afocal photos I'll put them up.
CE

Would like to see them...

BillT

That sounds really positive. Nice to get those chroma out of the way. I'm sure you noticed better overall definition as well. The nice thing is; you can get many of the benefits of an apochromat but at a lower N.A., so your sample and coverslip precision need not be any different than when using a .65 achromat. It was a focused and unique choice for Lomo to make an N.A. .65 apo. I don't think I have seen another one like it. Most are high N.A. with short w.d.'s and mostly, coverslip correction obligatory.