MicrobeHunter.com Microscopy Forum

You can also access this page with: www.microscopy-forum.com
It is currently Fri Aug 23, 2019 10:52 am

All times are UTC




Post new topic  Reply to topic  [ 17 posts ] 
Author Message
PostPosted: Wed Jun 26, 2019 7:33 pm 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
Hi all, I just received my Olympus BX40 and one of the eyepieces includes a (removable) reticle which, upon a quick search, turns out to be a "Walton/Becket" reticle, used for particle measurement & counting.
Here's an image of it,
Attachment:
ws_BX40 eyepiece reticle.jpg
ws_BX40 eyepiece reticle.jpg [ 22.4 KiB | Viewed 1245 times ]


I think this info is relevant, not sure..
Attachment:
ws_reticle with bx40.jpg
ws_reticle with bx40.jpg [ 29.4 KiB | Viewed 1245 times ]


It's 24mm diameter and is immaculate - I wonder if it may be useful?
Here's the actual reticle - apologies for poor image....
Attachment:
ws_walton becket reticle 24mm.jpg
ws_walton becket reticle 24mm.jpg [ 31.24 KiB | Viewed 1245 times ]


Does anyone know anything about it - what I may use it for if anything, or if I should sell it?

Thanks for any help or comments.

_________________
John B


Top
   
PostPosted: Wed Jun 26, 2019 9:10 pm 
Offline

Joined: Tue Dec 06, 2016 6:22 am
Posts: 568
Location: Santa Cruz, CA
The cross hairs might be useful to you? For:

- checking that head optics are aligned
- check parcentering of objectives
- maybe measuring things either using the stage rulers or adding a DRO


Top
   
PostPosted: Wed Jun 26, 2019 10:21 pm 
Offline

Joined: Mon Apr 10, 2017 8:24 am
Posts: 1344
Location: NorthWest England
.
https://www.pyseroptics.com/graticules/microscope-eyepiece-reticles/walton-beckett/
.
https://www.2spi.com/category/graticules-walton/

I suggest you either take-up Asbestos analysis, or sell it, John

MichaelG.

_________________
Too many 'projects'


Last edited by MichaelG. on Wed Jun 26, 2019 10:24 pm, edited 1 time in total.

Top
   
PostPosted: Wed Jun 26, 2019 10:24 pm 
Offline
User avatar

Joined: Sun Oct 12, 2014 2:34 am
Posts: 5947
Location: Estepona
According to this site they are worth around £130 new

https://www.pyseroptics.com/product/g22/

Concurrent posting!

_________________
Zeiss Standard WL on sick leave, Standard 18 & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)


Top
   
PostPosted: Wed Jun 26, 2019 10:38 pm 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
Thanks Michael, Pete & 75' - I think I may as well sell it - I can't see myself having a use for it really.

Thanks folks. I've put the BX40 together and it almost looks unused!
I'll post some images of it tomorrow. It has a couple of Achromats (x10 and x40) and a couple of the same magnification Phase objectives (Achromats also). It has the phase condenser, which is brighfield and darkfield capable too, beautifully made.

The performance is extremely good, especially considering these are the base-level Olympus UIS objectives - I'm very impressed indeed.

Back soon. :D

_________________
John B


Top
   
PostPosted: Thu Jun 27, 2019 6:28 am 
Offline

Joined: Mon May 06, 2019 11:44 am
Posts: 13
Location: Perth, Western Australia
Didn’t come with a HSE Test Slide did it?

_________________
I have a headache, For which the future’s made


Top
   
PostPosted: Thu Jun 27, 2019 8:51 pm 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
PrecisionInstruments wrote:
Didn’t come with a HSE Test Slide did it?


No, there was just the reticle......

_________________
John B


Top
   
PostPosted: Sun Jun 30, 2019 9:33 am 
Offline

Joined: Mon May 06, 2019 11:44 am
Posts: 13
Location: Perth, Western Australia
mrsonchus wrote:
PrecisionInstruments wrote:
Didn’t come with a HSE Test Slide did it?


No, there was just the reticle......


Bugger!! They’re worth a pretty penny

_________________
I have a headache, For which the future’s made


Top
   
PostPosted: Thu Jul 18, 2019 1:35 am 
Offline

Joined: Thu May 09, 2019 12:27 am
Posts: 21
The Walton Becket eyepiece reticle is use by Industrial Hygienist for Asbestos Fiber Counting. Keep in mind this is not Asbestos Identification, but counting of particles that are collected from the air.

Regards,
Wayne


Top
   
PostPosted: Thu Jul 18, 2019 5:34 am 
Offline

Joined: Mon Apr 10, 2017 8:24 am
Posts: 1344
Location: NorthWest England
p13 of this brochure has a little more information than the links I posted previously
https://issuu.com/pysersgi/docs/pyser-sgi_eyepiece_reticles/18?ff

MichaelG.

_________________
Too many 'projects'


Top
   
PostPosted: Thu Jul 18, 2019 7:48 am 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
That's the one.
It's no use to me so if anyone has a use for it just let me know, or I have it on flea-bay just to see if I can move it on...

Thanks all for your time.

John B.

_________________
John B


Top
   
PostPosted: Thu Jul 18, 2019 3:47 pm 
Offline
User avatar

Joined: Wed Nov 12, 2014 4:39 pm
Posts: 1815
Location: Illinois USA
mrsonchus wrote:
I've put the BX40 together and it almost looks unused!
I'll post some images of it tomorrow.


Nudge nudge ;)

_________________
Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope


Top
   
PostPosted: Thu Jul 18, 2019 6:00 pm 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
Crater Eddie wrote:
Nudge nudge ;)


Hi Ed! Ahhh, yes, really must put up some images. I've been that busy believe it or not I actually didn't think to do it! :oops:
I'll take some images tonight and 'put-em-up' my old friend.

John B

_________________
John B


Top
   
PostPosted: Thu Jul 18, 2019 8:33 pm 
Offline

Joined: Mon Nov 10, 2014 10:01 pm
Posts: 2872
Hello John B.,

Congrats on your new microscope!.. I am waiting to see photos of it and some subjects also...

The Best,
BillT


Top
   
PostPosted: Thu Jul 18, 2019 10:22 pm 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
Hi there Bill! I know - I really should have posted some images by now.

Here are a few images of the new BX40 - and when I say new, of course it's a 90s 'scope but actually hadn't so much as a fingerprint upon it - looked unused, or virtually-so.....

The BX40 I now have with Canon 200D atop.
Image

Various views around the beauty...
Image

Image

Image

Image

Image

Image

Image

Image

Image

Image

Image

Image

A few quick images through 'scope with Canon DSLR and Canon's 'Utility v3' tethering software.
An epidermal-peel I took today while preparing some pieces of Foxglove stem (and ovaries as it happens) for fixative (should make a nice set of slides I think) simply water-mounted with coverslip, brightfield.
I like the way this stack has enabled the focus to move into the interior of the stomatal-pore somewhat, very nice detail for a water-mount, nice and clear. So far so good...
Image

A stomate from the epidermis of a Daffodil ovary TS - again nice detail for a single image, especially of the continuation of the epidermal cuticle around the 'doors' of the pore. Rather nice detail also of cytoskeleton and vacuoles within cells. Stained with Alcian-blue and Safranin.
Image

I think these are Teliospores of rust-fungus, beneath the epidermis of Sonchus.arvensis.
Image

This is 40x phase-contrast at ludicrously-high ISO (the std 30W halogen barely manages especially with the use (as here) of a green filter, image in mono with camera. Stained slides aren't much use (nor are they intended to be of course) with phase-contrast, but all my current slides are stained... This is part of the vascular bundle (xylem vessels) of a Sonchus.oleraceus in TS.
Image

Here is the same in brightfield, far better as expected.... Above the xylem is a 2-3 cell thick cambium and above that the phloem vessels and their companion cells (which 'load' sugars and other substances into and out of the phloem tubes).
Image

LS along xylem vessels. The central vessel is a mature secondary vessel-element and shows typical 'bordered' (elliptical-elongate) pits in the (dead) secondary cell-walls. These vessels are formed after cell-elongation has finished as their rigid structure would cause them to fracture if they were formed earlier, during primary growth. The vessel-walls formed during primary growth need to be able to accommodate cell elongation stages and so will have helical or circular (lignin) bands that fulfil this requirement - a readily observable character that allows identification of primary vs secondary xylem.... The vessel that has been torn by the microtome knife, to the left of the bordered pitted vessel, is such a vessel with helical lignification...
Image

Oh yes, here's a rudimentary darkfield image of the aforementioned epidermal-peel (of a Foxglove stem) as provided by the 'DF' position of the BX40's phase contrast condenser. Not the best but definitely of use....
Image

That's about all I had time to put together this evening.

I love this BX40's clarity and sheer resolution of detail. I have a nice-looking (oh-my I really hope so......) UPlanApo DRY 60x 0.9 n.a. with correction-collar infinity objective arriving tomorrow which will hopefully serve me well as my 'high-dry' go-to before the dreaded 100x oil is needed. I'm hoping the correction-collar will help this objective to perform to it's best as the 60x dry objectives are horribly sensitive without one..... Fingers very firmly crossed - I'll post images when I get it up & running.....

More tomorrow hopefully....

John B.

_________________
John B


Last edited by mrsonchus on Thu Jul 18, 2019 10:59 pm, edited 1 time in total.

Top
   
PostPosted: Thu Jul 18, 2019 10:50 pm 
Offline

Joined: Mon Apr 10, 2017 8:24 am
Posts: 1344
Location: NorthWest England
O.K. ... I'm impressed !!
[and more than a little envious]

MichaelG.

_________________
Too many 'projects'


Top
   
PostPosted: Thu Jul 18, 2019 11:04 pm 
Offline
User avatar

Joined: Tue Feb 03, 2015 9:42 pm
Posts: 3449
Location: Cumbria, UK
Thanks Michael, it's a beauty!

I've moved (duplicated actually) this post over to a new thread of it's own - just realised this was originally about the graticle..... :oops:

John B.

_________________
John B


Top
   
Display posts from previous:  Sort by  
Post new topic  Reply to topic  [ 17 posts ] 

All times are UTC


Who is online

Users browsing this forum: No registered users and 4 guests


You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
Jump to:  
Powered by phpBB® Forum Software © phpBB Limited