Olympus MPLAN objectives

Everything relating to microscopy hardware: Objectives, eyepieces, lamps and more.
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perulero
Posts: 7
Joined: Sun May 05, 2019 4:47 am

Olympus MPLAN objectives

#1 Post by perulero » Fri Jul 19, 2019 6:40 am

Hi all

I’d like to get your opinion on olympus MPLAN objectives.
I have a few , can they be used for viewing biological protzoa
I know these were designed for metalurgical microscopy
Has anyone used them?

Joe

MichaelG.
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Joined: Mon Apr 10, 2017 8:24 am
Location: NorthWest England

Re: Olympus MPLAN objectives

#2 Post by MichaelG. » Fri Jul 19, 2019 6:54 am

As I think you already realise, Joe ...
'M' objectives are designed for use with no coverslip
... and are also not intended for dipping in water

The lower powers will probably be O.K. with a coverslip, but you are unlikely to get decent views with the high powers.

Specs. for the current range are here:
https://www.olympus-ims.com/en/microscope/mpln/

MichaelG
.

Edited to fix the URL
Last edited by MichaelG. on Fri Jul 19, 2019 4:45 pm, edited 2 times in total.
Too many 'projects'

perulero
Posts: 7
Joined: Sun May 05, 2019 4:47 am

Re: Olympus MPLAN objectives

#3 Post by perulero » Fri Jul 19, 2019 8:13 am

So can they be used to view tiny insects.minerals, plants
I have a 100x Mplan ,any possible use for it.?

Cheers

Joe

MichaelG.
Posts: 1514
Joined: Mon Apr 10, 2017 8:24 am
Location: NorthWest England

Re: Olympus MPLAN objectives

#4 Post by MichaelG. » Fri Jul 19, 2019 8:25 am

perulero wrote:So can they be used to view tiny insects.minerals, plants
I have a 100x Mplan ,any possible use for it.?
Radiolaria, maybe

http://www.radiolaria.org

MichaelG.
Individual radiolarians are normally in the size range of hundredths to tenths of millimeters, but some reach dimensions of a millimeter or more, large enough to be seen with the naked eye.
Too many 'projects'

PeteM
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Location: Santa Cruz, CA

Re: Olympus MPLAN objectives

#5 Post by PeteM » Fri Jul 19, 2019 3:28 pm

Your MPlan 100x might be OK for something like thin blood smears (no cover)? Since it's not meant to be used with oil (I believe, there may be variants), you'll find depth of field into a biological specimen even more problematic than usual.

I'd be curious if anyone knows what the difference in resolution and correction would be between a 4x Olympus UPlan Fl and a 5x Olympus MPlan Fl (beside the obvious difference in magnification)? Any difference quality-wise in pictures taken with these two? Both are fluorite optics. The Uplan has a N.A. of 1.3. The slightly higher mag MPlan Fl has a N.A. of 1.5. At 5x the MPlan still has plenty of depth of field to deal with a cover slip.

MichaelG.
Posts: 1514
Joined: Mon Apr 10, 2017 8:24 am
Location: NorthWest England

Re: Olympus MPLAN objectives

#6 Post by MichaelG. » Fri Jul 19, 2019 5:43 pm

PeteM wrote:I'd be curious if anyone knows what the difference in resolution and correction would be between a 4x Olympus UPlan Fl and a 5x Olympus MPlan Fl (beside the obvious difference in magnification)? < etc. >
A quick check on the Olympus sites:

https://www.olympus-lifescience.com/en/ ... es/uplfln/
https://www.olympus-ims.com/en/microscope/mplfln/

reveals that the two 'camps' display their specifications differently
and that the U claims excellence in a couple of areas, whilst the M claims nothing better than good.
... But there is no 'calibration' of these terms, and they may be working to different definitions !

Sorry ... I have never used either of them.

MichaelG.
Too many 'projects'

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