Everything looks better in slow motion
Everything looks better in slow motion
I found this in a sample of water, mixed with pollen, which I let sit out for like a week. I then took a sample of pollen biofilm type thing, and put it on a slide. The results were worth it.
No clue what this little guy is, BUT, I know that like everything, slow mo makes it better.
So without further ado, slow motion, of the cilia.
although, I think my ssd may have caused frameskipping, because it literally filled up in the middle of this and i need to deal with the 100 gb of microscopy footage on it somehow.
Anyways, on a side note, does anyone know what this ciliate is?
No clue what this little guy is, BUT, I know that like everything, slow mo makes it better.
So without further ado, slow motion, of the cilia.
although, I think my ssd may have caused frameskipping, because it literally filled up in the middle of this and i need to deal with the 100 gb of microscopy footage on it somehow.
Anyways, on a side note, does anyone know what this ciliate is?
Re: Everything looks better in slow motion
This is very impressive
… Keep up the good work
MichaelG.
… Keep up the good work
MichaelG.
Too many 'projects'
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Re: Everything looks better in slow motion
Very nice, enjoy these little clips. No idea what that is beyond saying it’s some type of peritrich. Perhaps someone more knowledgeable can offer more.
Keep them coming.
Harry
Keep them coming.
Harry
Re: Everything looks better in slow motion
Hi,
I noticed no more motion can be interesting also. Hawing looked at a live one, I was surprised by how it looked like after my slide ended drying up, by the length of cilias.
I may be wrong, other are welcome to comment, but usually, they seem to be described as being short. Here you see the difference of cilias length, of the same protozoa, my camera/video/setup not being able to catch the details at such high speeds:
I noticed no more motion can be interesting also. Hawing looked at a live one, I was surprised by how it looked like after my slide ended drying up, by the length of cilias.
I may be wrong, other are welcome to comment, but usually, they seem to be described as being short. Here you see the difference of cilias length, of the same protozoa, my camera/video/setup not being able to catch the details at such high speeds:
Re: Everything looks better in slow motion
That is very nice slow motion:-)
I would like to be able to do the same sort of thing.
Does the camera's letterbox view get taller when the frame rate is dropped ?
I would like to be able to do the same sort of thing.
Does the camera's letterbox view get taller when the frame rate is dropped ?
Re: Everything looks better in slow motion
More or less, tho the inverse is probably more accurate, as it gets larger, approaching 100% of the sensor, the fps gets lower.
Re: Everything looks better in slow motion
You make a very good point … but perhaps that ‘short’ description reveals that most previous observers have seen less detail than you have.
The motion of individual cilia is, I believe, rather like a whip-crack and therefore exceedingly difficult to image.
… I think you are already doing better than most !!
MichaelG.
Edit: _ there is an excellent sketch on this page:
https://www.sciencefacts.net/cilia-and-flagella.html
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Too many 'projects'
Re: Everything looks better in slow motion
Please forgive the slight digression, but this paper is rather impressive:
https://www.semanticscholar.org/paper/A ... b8209407e2
MichaelG.
https://www.semanticscholar.org/paper/A ... b8209407e2
MichaelG.
Too many 'projects'
Re: Everything looks better in slow motion
I was just lucky, it died (no more oxygen) in that position, showing me its cilias, at the border / outside of some remaining water. I was lucky and it was because I'm often looking at drying or even dried slides also.MichaelG. wrote: ↑Sun Mar 31, 2024 7:16 amYou make a very good point … but perhaps that ‘short’ description reveals that most previous observers have seen less detail than you have.
The motion of individual cilia is, I believe, rather like a whip-crack and therefore exceedingly difficult to image.
… I think you are already doing better than most !!
This was at 60x, were I have not much resolution, neither can I do a lot with oblique illumination here. So it should be complex to deliberately concentrate on thin cilias in common wet and live conditions.
I think cilias and flagellas movements are fast to very fast, so we mainly see their bases in normal observation conditions And in common representations, they are by the way all described as short, which could be their bases only?
Vorticellas are also fast, much bigger, more easy to observe, but they could need up to costly 1400FPS cameras to catch the details when they quickly retract...
There we can read flagellas have a passive part/base. If they are thin and fast, we probably won't see most of them, unless their bases.MichaelG. wrote: ↑Sun Mar 31, 2024 7:16 amEdit: _ there is an excellent sketch on this page:
https://www.sciencefacts.net/cilia-and-flagella.html
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Re: Everything looks better in slow motion
On the subject of cilia, I was lucky enough to capture these cilia, top left of the ciliate, in their "return" phase.
I'm guessing this image was taken at 1/100 or 1/80 sec.
Harry
I'm guessing this image was taken at 1/100 or 1/80 sec.
Harry
Re: Everything looks better in slow motion
That is a nice and interesting shot. On the right, cilias seem a bit blurry, less defined, I see two options for this:
- either those were a bit out of focus, out of depth of field
- or the speed of 1/100 or 1/80 was too slow to freeze their movements, movements causing blur, this being the advantage of sensitive and fast cameras
Using a camera like a DSLR, one may increase ISOs to reach lower exposure times. If possible (may add numerical noise), doubling ISOs allows to divide exposure time by two, thus freezing more the movements.
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Re: Everything looks better in slow motion
Thanks for the comments, bkt. I’ve got a flash adapter on the way to me that should help with many of my challenges.
Harry
Harry