Another Pediastrum

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75RR
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Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Another Pediastrum

#1 Post by 75RR » Tue Oct 28, 2014 2:09 am

40x, about 125µm, Offset Dark Stop, 5 image stack photoshop

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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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gekko
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Location: Durham, NC, USA.

Re: Another Pediastrum

#2 Post by gekko » Tue Oct 28, 2014 8:59 am

Very nice. I'm surprised that you get such a good result from stacking images taken with oblique illumination (I tried it a few times, and always my results were very disappointing).

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vasselle
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Re: Another Pediastrum

#3 Post by vasselle » Tue Oct 28, 2014 9:50 am

Bonjour
Très agréable jolie photo
Pourquoi vous utiliser pas le logiciel Combie CZM
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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75RR
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Re: Another Pediastrum

#4 Post by 75RR » Tue Oct 28, 2014 9:54 am

I'm surprised that you get such a good result from stacking images taken with oblique illumination ...
So am I. :)

A little more seriously, not having seen your efforts, I was wondering if the method I used Offset Dark Stop: I swing out the filter holder containing the Dark Stop, is more subtle than other methods and is therefore less of a challenge to use.

As an aside, I sorted out my WB on the video. Turns out the homemade diffuser I was using was too opaque.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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75RR
Posts: 8207
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Another Pediastrum

#5 Post by 75RR » Tue Oct 28, 2014 10:01 am

Pourquoi vous utiliser pas le logiciel Combie CZM
Have a Mac.

Will probably get zerene at some point.
For the moment photoshop works well enough for me.
I have not outgrown it yet :)
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Another Pediastrum

#6 Post by gekko » Tue Oct 28, 2014 3:45 pm

75RR wrote:
I'm surprised that you get such a good result from stacking images taken with oblique illumination ...
So am I. :)

A little more seriously, not having seen your efforts, I was wondering if the method I used Offset Dark Stop: I swing out the filter holder containing the Dark Stop, is more subtle than other methods and is therefore less of a challenge to use.

As an aside, I sorted out my WB on the video. Turns out the homemade diffuser I was using was too opaque.
Thank you. I use the same method as you-- it is the only way I can get oblique lighting, because with the Nikon condenser I cannot use a stop close to the plane of the condenser aperture to place any stops. I think I just don't have the knack for stacking :( .
Most homemade diffusers that I've used do affect the white balance, so I take a custom WB again after I put the diffuser in (unless I forget...)

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