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PostPosted: Wed Dec 05, 2018 8:29 pm 
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Joined: Sat Jun 18, 2016 11:55 am
Posts: 753
Location: Arnold, Missouri USA
OK, here are some better images.
Alanine 10x HMC
Image
Safranine 10x HMC
Image
OrangeG
Image
These look way more textured than Oblique and POL to me, but my peepers are early 1950s vintage.
Still learning.
Radazz

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Arnold, Missouri
Wild Heerbrugg M20
Olympus IX70 HMC
Olympus BX40 Phase Contrast
Nikon Labophot 2
Nikon Optiphot 66
Some Spencers and a few Bosch and Lombs


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PostPosted: Wed Dec 05, 2018 8:41 pm 
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Location: Germany, Karlsruhe
Indeed great images Radazz!
Would be nice to have the comparison Oblique + Pol versus HMC.

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sincerely Jochen
My microscopes: Zeiss inverse IM35, Zeiss Standard (RA, WL, Universal, Junior), Stemi III
Youtube: https://www.youtube.com/channel/UCVio3U ... cg-Fm2su3w


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PostPosted: Wed Dec 05, 2018 10:23 pm 
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So jealous. Maybe I will take the time to make my own HMC slit plates.

mnmyco


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PostPosted: Thu Dec 06, 2018 2:09 am 
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ImperatorRex wrote:
Indeed great images Radazz!
Would be nice to have the comparison Oblique + Pol versus HMC.

Interesting, something for the next session.
Thanks, Dominus,
Radazz

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Arnold, Missouri
Wild Heerbrugg M20
Olympus IX70 HMC
Olympus BX40 Phase Contrast
Nikon Labophot 2
Nikon Optiphot 66
Some Spencers and a few Bosch and Lombs


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PostPosted: Thu Dec 06, 2018 3:05 am 
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Location: Santa Cruz, CA
Gorgeous images.

If you get a chance to compare alternative methods -- would be very interested to see them.


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PostPosted: Thu Dec 06, 2018 1:32 pm 
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Hi Radazz,
thank you for showing these nice images! I too would like to see a comparison of this rare contrast system with pol + oblique combined with pol.

I would also like to see an image of a typical phase contrast-object in phase and Hoffman contrast if possible.

The Hoffman system works with assymetrical stops - is there preferred way to orient them to the object and it's structures?

This might be a system that is uncommon but might as well have had more success.

Bob


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PostPosted: Thu Dec 06, 2018 4:37 pm 
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Joined: Thu Aug 23, 2018 10:12 pm
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Nice to see this thread! I just picked up a Nikon 40x 0.55 HMC LWD objective with correction collar pretty cheap on eBay. Got three Nikon CF objectives for a grand sum of £75: the 40x HMC objective, a plan achromat 20x 0.40 ph2 phase objective with correction collar, and a simple E achromat 10x 0.25 ph1 objective. Worth a shot, I thought. The only phase objective I had previously was a 40x CF N plan objective (and a Heine condenser) plus some WI Lomo's, so I got two more phase objectives that will be useful to me, plus a completely new illumination technique!

I don't have a HMC condenser and I don't plan to get one as I'm using my Nikon objectives on a Wild M20 stand, but based on what I've read it seems like it should be possible to make a DIY version by making a slit anullus and placing it in an empty slot in my phase condenser. Getting the slit to line up with the modular plate in the objective might be tricky without being able to rotate it easily, but should be doable.

I'll post some results when I have some (but it might take a while before I have time to do that).


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PostPosted: Thu Dec 06, 2018 5:59 pm 
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Hi viktor,
you might print a black disc with multiple slits with a laser printer on transparency film and get a better idea where the slit has to be.
I'm looking forward for your project updates.

Bob


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PostPosted: Thu Dec 06, 2018 6:16 pm 
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Joined: Thu Aug 23, 2018 10:12 pm
Posts: 11
Hi Bob,
My idea was similar: to print a fine grid system on transparent film to see where the slit should be.

Looking at: https://micro.magnet.fsu.edu/primer/tec ... intro.html

The simplest design that could work is simply a single slit, as in figure 4. But I think it would be simple to add some polarizing film that covers half the slit and place a rotating polarizer below the condenser so as to manipulate the width of the slit, as in Figure 2.

Fun project, this.


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PostPosted: Fri Dec 07, 2018 12:51 am 
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Location: Arnold, Missouri USA
MicroBob wrote:
Hi Radazz,
thank you for showing these nice images! I too would like to see a comparison of this rare contrast system with pol + oblique combined with pol.

I would also like to see an image of a typical phase contrast-object in phase and Hoffman contrast if possible.

The Hoffman system works with assymetrical stops - is there preferred way to orient them to the object and it's structures?

This might be a system that is uncommon but might as well have had more success.

Bob

Not enough room to set up both the HMC and Phase contrast scopes so I can't do a side by side of Ph and HMC, but I've got some PH shots to compare.
The HMC, POL, oblique set will be easy, its on the list.
Radazz

_________________
Arnold, Missouri
Wild Heerbrugg M20
Olympus IX70 HMC
Olympus BX40 Phase Contrast
Nikon Labophot 2
Nikon Optiphot 66
Some Spencers and a few Bosch and Lombs


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