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 Post subject: Diatoms
PostPosted: Fri Dec 07, 2018 12:43 am 
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Joined: Sat Jun 18, 2016 11:55 am
Posts: 926
Location: Arnold, Missouri USA
These are better
Olympus IX70 HMC

20x obj
Seven image stack (picoley)
Image

40x obj
Image

Enjoy,
Radazz

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Arnold, Missouri
Olympus IX70 HMC
Olympus BX40 Phase Contrast
Olympus SZ40 Stereoscope


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 Post subject: Re: Diatoms
PostPosted: Fri Dec 07, 2018 7:52 am 
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Joined: Mon Dec 03, 2018 7:05 pm
Posts: 130
Location: Huizen, Netherlands
Beautifull! I am new to microscopy and amazed about what's possible.


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 Post subject: Re: Diatoms
PostPosted: Fri Dec 07, 2018 8:56 am 
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Joined: Mon Aug 21, 2017 9:02 pm
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Attractive creation!

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Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo


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 Post subject: Re: Diatoms
PostPosted: Sat Dec 08, 2018 3:47 pm 
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Joined: Sat Jun 20, 2015 3:57 am
Posts: 2653
Location: Houston, Texas
Very nice, thank you for sharing!

I would like to see a diatom comparison between HMC and offset oblique, if possible. A separated flat Gyrosigma would work well for that comparison.

Looking at the above Gyrosigma in HMC though, it does seem that HMC may have reduced aperture by too much, such that dot resolution is lost. I am only guessing based on Gyrosigma experience though and could totally be wrong.


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 Post subject: Re: Diatoms
PostPosted: Sat Dec 08, 2018 4:13 pm 
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Joined: Thu Aug 03, 2017 10:28 am
Posts: 107
Location: UK
zzffnn wrote:
Very nice, thank you for sharing!

I would like to see a diatom comparison between HMC and offset oblique, if possible. A separated flat Gyrosigma would work well for that comparison.

Looking at the above Gyrosigma in HMC though, it does seem that HMC may have reduced aperture by too much, such that dot resolution is lost. I am only guessing based on Gyrosigma experience though and could totally be wrong.


Could it be that the stacking process has spoilt the resolution of the dots here?


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 Post subject: Re: Diatoms
PostPosted: Sat Dec 08, 2018 5:57 pm 
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Joined: Sat Jun 18, 2016 11:55 am
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Location: Arnold, Missouri USA
That would be my guess.

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Arnold, Missouri
Olympus IX70 HMC
Olympus BX40 Phase Contrast
Olympus SZ40 Stereoscope


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 Post subject: Re: Diatoms
PostPosted: Sat Dec 08, 2018 8:27 pm 
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Joined: Thu Aug 03, 2017 10:28 am
Posts: 107
Location: UK
I thought I would see what I could do with my home-made HMC set-up. By home made I mean that both the insert in the condenser and that in the objective have been fabricated by me, quite simply with fairly basic materials. I realise that it is not ideal, and needs some tinkering with, but then that is part of the enjoyment.

Unfortunately, I read the name Gyrosigma in the text of a comment, and quite missed the fact that I think the sigmoidal frustule in the original shot is Pleurosigma. Hopefully it still illustrates if it works OK.

Attachment:
Gyrosigma HMC.jpg
Gyrosigma HMC.jpg [ 43.32 KiB | Viewed 977 times ]


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 Post subject: Re: Diatoms
PostPosted: Sun Dec 09, 2018 6:28 am 
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Location: Houston, Texas
My mistake, sorry about that.

A side by side comparison of HMC vs oblique would be nice.


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 Post subject: Re: Diatoms
PostPosted: Sun Dec 09, 2018 4:48 pm 
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Joined: Sat Jun 18, 2016 11:55 am
Posts: 926
Location: Arnold, Missouri USA
I’m going to have to set up the BX40 next to the IX70 to make this comparison.
Still trying to figure out how to do darkfield or oblique on the inverted.
I ordered some acrylic disks to fit the filter carrier on the illuminator in order to try creating some stops.
However, the Silly Season is using much more time than usual, and I will try after the holidays.

Bare with me,
Radazz

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Arnold, Missouri
Olympus IX70 HMC
Olympus BX40 Phase Contrast
Olympus SZ40 Stereoscope


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 Post subject: Re: Diatoms
PostPosted: Thu Dec 13, 2018 8:30 am 
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Joined: Thu Aug 23, 2018 10:12 pm
Posts: 51
photomicro wrote:
I thought I would see what I could do with my home-made HMC set-up. By home made I mean that both the insert in the condenser and that in the objective have been fabricated by me, quite simply with fairly basic materials. I realise that it is not ideal, and needs some tinkering with, but then that is part of the enjoyment.

Unfortunately, I read the name Gyrosigma in the text of a comment, and quite missed the fact that I think the sigmoidal frustule in the original shot is Pleurosigma. Hopefully it still illustrates if it works OK.

Gyrosigma HMC.jpg


Nice! Have you described your home-made HMC setup somewhere? Since I am going to build my own HMC condenser, I'd love to see it and get some ideas. I'm impressed you built your own objective, too. Was it difficult to get the modulator at the correct position at the objective's back focal plane? I know Leica has a system where the modulator sits outside the objective (Leica Integrated Modulation Contrast), but I would guess that that is only possible with infinity corrected optics.


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 Post subject: Re: Diatoms
PostPosted: Thu Dec 13, 2018 8:02 pm 
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Joined: Fri Mar 30, 2018 4:12 pm
Posts: 169
Location: Germany, Karlsruhe
Hi Photomicro,
looks good. What Objective (n.A.) have you used with your HMC? Your test diatoma is Gyrosigma and the structure just is partly resolved, so I would assume an objective with a n.A. of something like 0.40?

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sincerely Jochen
My microscopes: Zeiss inverse IM35, Zeiss Standard (RA, WL, Universal, Junior), Stemi III
Youtube: https://www.youtube.com/channel/UCVio3U ... cg-Fm2su3w


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