Leukocyte crawling in blood

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Wes
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Leukocyte crawling in blood

#1 Post by Wes » Sun Oct 18, 2020 5:32 pm



Sped up 4x, 40x/0.95 DIC with 2x optovar magnification.

Maybe I can add some bacteria or yeast to the blood and see if they react to it.

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75RR
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Re: Leukocyte crawling in blood

#2 Post by 75RR » Sun Oct 18, 2020 6:10 pm

Super video!
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Hobbyst46
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Re: Leukocyte crawling in blood

#3 Post by Hobbyst46 » Sun Oct 18, 2020 6:30 pm

Great !
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DonSchaeffer
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Re: Leukocyte crawling in blood

#4 Post by DonSchaeffer » Sun Oct 18, 2020 7:45 pm

Creepy enough for a monster movie!

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janvangastel
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Re: Leukocyte crawling in blood

#5 Post by janvangastel » Sun Oct 18, 2020 8:52 pm

Beautifull!!

tgss
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Re: Leukocyte crawling in blood

#6 Post by tgss » Sun Oct 18, 2020 9:05 pm

Very, very interesting indeed to see that. Thanks for sharing.
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Javier
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Re: Leukocyte crawling in blood

#7 Post by Javier » Mon Oct 19, 2020 12:48 am

That is awesome!!

Wes
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Re: Leukocyte crawling in blood

#8 Post by Wes » Mon Oct 19, 2020 7:04 am

Thank you all for the interest and comments.

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Re: Leukocyte crawling in blood

#9 Post by DonSchaeffer » Mon Oct 19, 2020 10:19 pm

That's a white blood cell right? Then it's not as creepy as I thought.

Wes
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Re: Leukocyte crawling in blood

#10 Post by Wes » Tue Oct 20, 2020 6:14 am

DonSchaeffer wrote:
Mon Oct 19, 2020 10:19 pm
That's a white blood cell right? Then it's not as creepy as I thought.
Yes, probably a neutrophil. Someone asked me in the youtube comments section what are the small fast moving things. Someone else suggested platelets, initially I thought it may be but then I checked again and platelets were not as numerous and small as the particles so any further suggestions are welcome.

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Re: Leukocyte crawling in blood

#11 Post by ImperatorRex » Tue Oct 20, 2020 5:04 pm

Wes,
impressive video for certain. When I saw the Leukocyte I first though it looks like an amobea? And the small little things moving arround would be just bacteria?
But this would then mean that the blood sample got contaminated with an other sample. Just thoughts and I have really to admit that I have no idea how Leukocytes actually look like.

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Re: Leukocyte crawling in blood

#12 Post by Wes » Wed Oct 21, 2020 3:57 pm

Thank you Jochen1
ImperatorRex wrote:
Tue Oct 20, 2020 5:04 pm
And the small little things moving arround would be just bacteria?
This was a fresh blood sample from my finger. If these were bacteria I would've been very sick at this point and I felt better than ever ;) I suspect its dirt from the slide/cover slip although I don't see this in pond samples but I've seen the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.

In phase contrast I could observe extremely fine fibers, I think made from the protein fibrin that clots blood.

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ImperatorRex
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Re: Leukocyte crawling in blood

#13 Post by ImperatorRex » Wed Oct 21, 2020 4:35 pm

Wes,
how did you actually prepare the slide? Wonder if you added other ingredience, for example an isotonic saline solution? Maybe this was contaminated over the time?

the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.

This is really interesting!

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Re: Leukocyte crawling in blood

#14 Post by Wes » Wed Oct 21, 2020 5:11 pm

ImperatorRex wrote:
Wed Oct 21, 2020 4:35 pm
Wes,
how did you actually prepare the slide? Wonder if you added other ingredience, for example an isotonic saline solution? Maybe this was contaminated over the time?

the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.

This is really interesting!
I took a needle, pricked my finger and deposited a small drop of blood on a coverslip that I then added to a slide. No isotonic saline, as simple as you can picture it.

I just checked a bunch of DIC blood videos on youtube and I see the exact same particles. So I say blood specific thing...maybe platelet particles/chunks or cellular debri?

EYE C U
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Re: Leukocyte crawling in blood

#15 Post by EYE C U » Wed Oct 21, 2020 7:52 pm

https://youtu.be/4L5XMMlDGTA

AFRAID TO ASK WHAT IS CRAWLING AROUND...

Bruce Taylor
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Re: Leukocyte crawling in blood

#16 Post by Bruce Taylor » Wed Oct 21, 2020 9:48 pm

ImperatorRex wrote:
Tue Oct 20, 2020 5:04 pm
When I saw the Leukocyte I first though it looks like an amobea?
In a very real sense, leukocytes are amoebae. Cells that use amoeboid (myosin-mediated) movement are quite common among animals, and the ancestor we share with our nearest single-celled relatives (choanoflagellates, filastereans, etc.) likely had an amoeboid life phase, as well: https://bmcbiol.biomedcentral.com/artic ... 020-0762-1. On the eukaryotic tree of life, we happen to share a large branch with Amoebozoa--a broad clade called Amorphea, made up of amoebozoans, animals, fungi and some others. So, it's not too surprising that we share some cellular phenotypes with our little amoeba-cousins. :D

The video is just stunning, Wes!

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Re: Leukocyte crawling in blood

#17 Post by Wes » Thu Oct 22, 2020 10:52 am

Bruce Taylor wrote:
Wed Oct 21, 2020 9:48 pm
ImperatorRex wrote:
Tue Oct 20, 2020 5:04 pm
When I saw the Leukocyte I first though it looks like an amobea?
In a very real sense, leukocytes are amoebae. Cells that use amoeboid (myosin-mediated) movement are quite common among animals, and the ancestor we share with our nearest single-celled relatives (choanoflagellates, filastereans, etc.) likely had an amoeboid life phase, as well: https://bmcbiol.biomedcentral.com/artic ... 020-0762-1. On the eukaryotic tree of life, we happen to share a large branch with Amoebozoa--a broad clade called Amorphea, made up of amoebozoans, animals, fungi and some others. So, it's not too surprising that we share some cellular phenotypes with our little amoeba-cousins. :D

The video is just stunning, Wes!
Thank you Bruce for the comments and interesting paper that you linked reminding us of our distant relatives :D

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Re: Leukocyte crawling in blood

#18 Post by Bruce Taylor » Thu Oct 22, 2020 3:24 pm

Wes wrote:
Thu Oct 22, 2020 10:52 am
Thank you Bruce for the comments and interesting paper that you linked reminding us of our distant relatives :D
Not so distant, in the scheme of things. ;) Here's the eukaryote tree, from a recent classification:
Euk tree.png
Euk tree.png (179.67 KiB) Viewed 364 times
Image created by Yana Eglit for: https://onlinelibrary.wiley.com/doi/ful ... /jeu.12691

We're in Holozoa at the upper right, cuddled up to Nucletmycea (Fungi, Nuclearia, etc.), just downstream from the branching of Amoebozoa.

(Of course, this only includes complex cells; most of the world's diversity consists of bacteria and archaea)

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Re: Leukocyte crawling in blood

#19 Post by westy » Fri Oct 23, 2020 3:56 pm

Amazing video!
Ben

Wes
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Re: Leukocyte crawling in blood

#20 Post by Wes » Sat Oct 24, 2020 6:26 pm

Bruce Taylor wrote:
Thu Oct 22, 2020 3:24 pm
Wes wrote:
Thu Oct 22, 2020 10:52 am
Thank you Bruce for the comments and interesting paper that you linked reminding us of our distant relatives :D
Not so distant, in the scheme of things. ;) Here's the eukaryote tree, from a recent classification:

Euk tree.png
Image created by Yana Eglit for: https://onlinelibrary.wiley.com/doi/ful ... /jeu.12691

We're in Holozoa at the upper right, cuddled up to Nucletmycea (Fungi, Nuclearia, etc.), just downstream from the branching of Amoebozoa.

(Of course, this only includes complex cells; most of the world's diversity consists of bacteria and archaea)
Thank you Bruce you for helping me develop a better appreciation of our evidently not-so-distant amoeboid cousins. Now I feel humbled when an amoeba crosses the field of view ;)
westy wrote:
Fri Oct 23, 2020 3:56 pm
Amazing video!
Very kind of you westy, thanks!

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