Marine nematode

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75RR
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Marine nematode

#1 Post by 75RR » Thu Sep 17, 2020 12:02 pm

Zeiss Plan 40x/0.65, DIC, stitched and stacked in Photoshop, Marine sample, Alboran Sea.

Note damage towards tail, might have occurred when I pressed the cover slip down.
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janvangastel
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Re: Marine nematode

#2 Post by janvangastel » Thu Sep 17, 2020 2:01 pm

Detailed and sharp as ussual 75RR. Very nice photograph.

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75RR
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Re: Marine nematode

#3 Post by 75RR » Thu Sep 17, 2020 4:11 pm

Many thanks janvangastel
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KD Arvidsson
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Re: Marine nematode

#4 Post by KD Arvidsson » Fri Sep 18, 2020 10:03 pm

Very beautiful and sharp 75RR//KD
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Wes
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Re: Marine nematode

#5 Post by Wes » Sat Sep 19, 2020 4:47 am

Really nice shot.

I don't understand how you make such good stitches, especially of a nematode that I'd expect to jiggle around.
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75RR
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Re: Marine nematode

#6 Post by 75RR » Sat Sep 19, 2020 5:47 am

Many thanks KD Arvidsson and Wes
I don't understand how you make such good stitches, especially of a nematode that I'd expect to jiggle around.
Mostly patience. There is an ideal window between a pinned nematode/diatom etc ... and a drying out cover slip.

This only works if there is nothing large under the cover slip stopping it from flattening out nicely.

I usually pick up a small bit of seaweed with tweezers and tap it lightly on the slide before returning it to the sample jar.

Best slides are those where 'by eye' it would seem that I did not transfer anything from the sample jar but water.

I say seems, there are always several somethings!
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viktor j nilsson
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Re: Marine nematode

#7 Post by viktor j nilsson » Sat Sep 19, 2020 6:52 am

75RR wrote: I usually pick up a small bit of seaweed with tweezers and tap it lightly on the slide before returning it to the sample jar.

Best slides are those where 'by eye' it would seem that I did not transfer anything from the sample jar but water.
This is really good advice which deserves to be repeated. I've found that really hard to learn, and always end up with too much gunk on my slide.

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