60x, 100x objective + dic
Living Diatom
Re: Living Diatom
Good video, looks like Stauroneis.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
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ImperatorRex
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Re: Living Diatom
Thanks for sharing this nice video!
Re: Living Diatom
Nice!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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janvangastel
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Re: Living Diatom
Sharp and detailed vido.
Re: Living Diatom
Beautiful observations of a living diatom, I love those green hues, thank you. Can you tell us how difficult...or not difficult it is to use a live organism wet-mount slide with transmitted DIC illumination?
Is the 'tight working distance of: 60X and 100X DIC objectives" a tricky and tedious challenge to observing wetmount slideswith these magnifications?
I also ask why yout list: "60x and 100X DIC" for this video...is this diatom really that small in size that both 60X and 100X objectives capture almost this sweet organisms entire body length?
I really enjoy this live diatiom observation you share...and I love the sounds of gurrgeling water you included! charlie guevara, fingerlakes/US
Is the 'tight working distance of: 60X and 100X DIC objectives" a tricky and tedious challenge to observing wetmount slideswith these magnifications?
I also ask why yout list: "60x and 100X DIC" for this video...is this diatom really that small in size that both 60X and 100X objectives capture almost this sweet organisms entire body length?
I really enjoy this live diatiom observation you share...and I love the sounds of gurrgeling water you included! charlie guevara, fingerlakes/US
Re: Living Diatom
Amazing!
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LouiseScot
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Re: Living Diatom
Like it! And the sound of running water 
Louise
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Living Diatom
It’s not that hard.charlie g wrote: ↑Wed Mar 03, 2021 3:24 pmBeautiful observations of a living diatom, I love those green hues, thank you. Can you tell us how difficult...or not difficult it is to use a live organism wet-mount slide with transmitted DIC illumination?
Is the 'tight working distance of: 60X and 100X DIC objectives" a tricky and tedious challenge to observing wetmount slideswith these magnifications?
I also ask why yout list: "60x and 100X DIC" for this video...is this diatom really that small in size that both 60X and 100X objectives capture almost this sweet organisms entire body length?
I really enjoy this live diatiom observation you share...and I love the sounds of gurrgeling water you included! charlie guevara, fingerlakes/US
But we need more effort to make clean and thin wet-mount slides.
Because dic is highly influenced by the thickness of the wet-mount slide at high magnification.
Dic isn't the way it works in every case. Sometimes brightfield, phase contrast is more suitable than dic.
Of course, it's just a personal opinion.
Obviously, in most cases, dic is much suitable better than phase contrast, brightfield.
Phase contrast is extremely limited. but, phase contrast shows better contrast than dic.
Of course, we need make extremely thin wet-mount slides for phase contrast.
This diatom is small.
I used 60x 100x objective with 2.5x photo eyepiece.
This vid show One-third of the area I can see through super wide-field eyepiece(26.5 mm).
I'm sorry, I'm not good at English.
Thanks, Charlie.
Re: Living Diatom
Your english is good. I understood everything.
Video is quite good also, lots of detail.
Video is quite good also, lots of detail.