Hydrodictyon in Different Lighting

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JimT
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Hydrodictyon in Different Lighting

#1 Post by JimT » Thu Jul 23, 2015 6:25 pm

Ala Gekko's Haematococcus posting of illumination effects I decided to do a similar experiment. Not as good as his images but interesting illustration of different lighting methods.

All with Canon 60D, eyepiece projection, and 10x obj.

1 is bright field
Image
2 is dark field
Image
3 is oblique
Image
4 is my DIY DIC filter
Image
5 is polarized
Image

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mrsonchus
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Re: Hydrodictyon in Different Lighting

#2 Post by mrsonchus » Thu Jul 23, 2015 6:39 pm

Excellent images, the last one is particularly interesting, it suggests (being polarized) structural information of the 'joins' between cells - very interesting to closely examine this region...
Thanks for a super post - It's making me consider fitting a polarizer to my 'scope... :)
John B

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Crater Eddie
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Re: Hydrodictyon in Different Lighting

#3 Post by Crater Eddie » Thu Jul 23, 2015 9:56 pm

All gorgeous photos, and a great find! I have never come across a sample of the "water net".
CE
Olympus BH-2 / BHTU
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)
Cameras: Canon T3i, Olympus E-P1 MFT, Amscope 3mp USB

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gekko
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Re: Hydrodictyon in Different Lighting

#4 Post by gekko » Thu Jul 23, 2015 10:00 pm

Beautiful! Both the images with different lighting methods, as well as the composition showing the perfect geometry. Lovely algae too.
JimT wrote:Ala Gekko's Haematococcus posting of illumination effects I decided to do a similar experiment. Not as good as his images but interesting illustration of different lighting methods.
I very much appreciate the complement, and you are absolutely right: not as good, but much better, and I really mean it.

JimT
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Re: Hydrodictyon in Different Lighting

#5 Post by JimT » Thu Jul 23, 2015 11:07 pm

Thank you John B. An easy way to get started with polarization is to get a pair of 3D glasses from your local movie house, break the lenses out, cut one to fit inside the microscope head and place the other one below the specimen. If your lamp doesn't get hot you can place it right on top the light.

CE, thanks. Maybe I should send you some of this pond water :)

Gekko, thanks. I don't think these were that good because of the difficult DOF (even with the stacking) and certainly not better than yours.

It was a fun project and I will probably do it again with other algae.

JimT

apochronaut
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Re: Hydrodictyon in Different Lighting

#6 Post by apochronaut » Fri Jul 24, 2015 1:32 am

It is truly remarkable, just how much of nature is structured with a hexagon. Very interesting post and pictures.

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vasselle
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Re: Hydrodictyon in Different Lighting

#7 Post by vasselle » Fri Jul 24, 2015 6:47 pm

Bonjour.
Superbe.
Cordialement seb
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Boitier EOS 1200D + EOS 1100D

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zzffnn
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Re: Hydrodictyon in Different Lighting

#8 Post by zzffnn » Fri Jul 24, 2015 7:18 pm

Very nice photos. Thank you for sharing.

SteveSteve
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Re: Hydrodictyon in Different Lighting

#9 Post by SteveSteve » Fri Jul 24, 2015 7:34 pm

Great photographs and really interesting to see the different lighting conditions.

What a beautiful species that is!

Cheers
Steve

JimT
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Re: Hydrodictyon in Different Lighting

#10 Post by JimT » Fri Jul 24, 2015 7:58 pm

Thanks all for the gracious comments.

charlie g
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Re: Hydrodictyon in Different Lighting

#11 Post by charlie g » Fri Jul 24, 2015 11:48 pm

As already said...beautiful algae you give here...thank you,Jim T. I'm fond of your DF image...those marvelous struts, the symetries...it's like heading to dock at a space station,Jim T ...beautiful images...charlie guevara

JimT
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Re: Hydrodictyon in Different Lighting

#12 Post by JimT » Sat Jul 25, 2015 3:15 pm

Thank you Charlie

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gekko
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Re: Hydrodictyon in Different Lighting

#13 Post by gekko » Sat Jul 25, 2015 9:25 pm

JimT wrote:Gekko, thanks. I don't think these were that good because of the difficult DOF (even with the stacking)
I think the limited depth of field is a decided plus here, as it suggests very clearly the 3-mimntional nature of the "structure". Beautiful!

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