Diplophrys (Labyrinthulomycota)

[Wes]

Captured via the floating coverslip method. You can see two opposing tufts of anastomozing pseudopodia, a large oil droplet and a nucleus adjacent to it.

[Microscopy_is_fun]

Interesting organism! Which contrast method did you use for this picture?

[Wes]

Interesting organism! Which contrast method did you use for this picture?

DIC

[Francisco]

Very nice!

[tlansing]

Nice photo, Wes. I have some coverglasses floating right now so I'll see what I find. Do you put them back if they haven't developed enough?

I was curious that the oil drop seen in this amoeba is not colored, as is commonly the case.

Tim

[Wes]

Thank you Tim and Francisco

Do you put them back if they haven't developed enough?

Yes, even if they are well developed I still return them after I'm done observing them.

I was curious that the oil drop seen in this amoeba is not colored, as is commonly the case.

You are correct, most often I find ones with yellow-brown droplets but this ones was less intensely colored.

[ImperatorRex]

Hi Wes,
nice catch! Your photos show a nice contrast of the filopodia.

There are currently 3-4 species of Diplophrys described, so there is a Diplophrys species whose lipid droplets are colorless - maybe you catched this one
I found the shape of the filopodia do change frequently... Did you also observed such changes? (see illustration below)
Some of the different forms I tried to document here: https://www.mikro-tuemplerforum.at/view ... hrys#p1899

[Wes]

Nicely documented thread Jochen, thanks for the link. Perhaps the species I found was in fact the one with colorless oil, and yes the filopodia did move slowly I'd say it looked as if they are used for crawling on the surface of the coverslip.