revisiting Diatomeen Ugchelen 6

[75RR]

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revisiting Diatomeen Ugchelen 6
viewtopic.php?f=6&t=13181&p

Stauroneis angustilancea 85µm and 2 diatoms in Girdle view at 65µm and 40µm that could be Eunotia sp.

Corrections as always are welcome ...

Zeiss Planapo 63x/1.4, DIC, stacked in Photoshop, camera set to Monotone
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[tlansing]

Beautiful photos!

[LouiseScot]

I love a nice hi-res diatom! Any chance of a link to larger versions?

Happy New Year!

Louise

[75RR]

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Thanks tlansing and LouiseScot

Any chance of a link to larger versions?

Don't have any but made a bigger jpeg version from the original 45 image stack TIFF of the Eunotia 65µm.

Pretty close to empty magnification at this size
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[Hobbyst46]

All are very nice, and indeed the latter ("Bigger") Eunotia shows even better contrast and resolution than the former ! I enlarged them many folds to inspect...

[75RR]

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Many thanks Hobbyst46,

Have gone back to the Projektiv 63mm which I am now trying to fine tune.

Given that my camera, due to its excess vibration is on a tripod, and as I don't have a permanent spot for my microscope at the moment (it hot-bunks desk space with my monitor and keyboard),

means that using a Projection eyepiece (no lens on the camera) entails setting up the camera at the correct parfocal distance every time I use the microscope.

This is as you can imagine, a little hit and miss.

The advantage of the Projectiv 63mm which is afocal (requiring a camera lens) is that it transmits a parallel beam, so setting up the camera is painless and consistent, once the Projectiv is dialed in.

Will just have to live with the hot-spot for now.