Closterium sp.

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Seb28
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Closterium sp.

#1 Post by Seb28 » Tue Nov 04, 2014 12:48 pm

Hi everyone.
Closterium sp. in autofluorescence,Neofluar 40/0.75,Zeiss Photomicroscope ll.

ImageDSCN0212.jpg-a by microx2013, on Flickr

ImageDSCN0185 by microx2013, on Flickr
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
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JimT
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Re: Closterium sp.

#2 Post by JimT » Tue Nov 04, 2014 3:02 pm

Interesting image Vs. white light. JimT
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75RR
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Re: Closterium sp.

#3 Post by 75RR » Tue Nov 04, 2014 3:21 pm

Very nice.
Did you also take some photos with another form of illumination?
I find the comparison of different techniques on the same subject interesting.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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vasselle
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Re: Closterium sp.

#4 Post by vasselle » Tue Nov 04, 2014 6:40 pm

Hello
Superbe images
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D
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Manfred
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Re: Closterium sp.

#5 Post by Manfred » Tue Nov 04, 2014 7:05 pm

Top equipment, fascinating image
Best regards
Manfred
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Oliver
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Re: Closterium sp.

#6 Post by Oliver » Tue Nov 04, 2014 7:51 pm

Seb,
What lighting did you use? Did you use UV light and a dark-field filter? I tried it with blue light LEDs but without much success. The wavelength was, evidently, not correct.
Thanks,
Oliver.
Image Oliver Kim - http://www.microbehunter.com - Microscopes: Olympus CH40 - Olympus CH-A - Breukhoven BMS student microscope - Euromex stereo - uSCOPE MXII
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gekko
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Re: Closterium sp.

#7 Post by gekko » Wed Nov 05, 2014 10:31 am

Hi seb28,
As Oliver said. Lovely images, as usual. Congratulations on your new microscope and fluorescence capability!
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Seb28
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Re: Closterium sp.

#8 Post by Seb28 » Fri Nov 07, 2014 5:43 am

Thank you for your comments.

75RR,
I only have one image as a comparison.
Untitled-1m.jpg
Untitled-1m.jpg (119.25 KiB) Viewed 5305 times
Oliver
The Zeiss photomicroscope have a fluorescence condenser with filters and dichronic mirrors for observing in Ultar violet,violet,blue and green excitation light.I use it with 10w LED or 60w 12V bulb.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
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75RR
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Re: Closterium sp.

#9 Post by 75RR » Fri Nov 07, 2014 9:33 am

Thanks for the alternate image. It provides a very good illumination comparison.
I use it with 10w LED or 60w 12V bulb.
Am trying to convert my Zeiss 60w Illuminator to led at the moment (forced conversion - it is missing some parts and not working),
can you provide details: photos/drawings etc... + comments on how it compares to the 60w bulb? Thanks
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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