40X and 60X objectives

[JimT]

Here is a lateral section of a celery stark Stomata, iodine stained with the 40x obj. and my new 60x obj. I promised to give my opinion and thanks to gekko for the suggestion to go for the obj. VS. two 15x eyepieces.

First observation is that the distance to the specimen is enough less than the 40x obj so it took me several attempts to cut the celery thin enough. Second observation is that it is a 50% increase in "real" magnification without having to resort to my 100x OI obj.

Anyway, very pleased with the obj. JimT

[75RR]

Hi JimT,
can you provide some info on the 40x and 60x objectives?

[gekko]

Thank you, JimT. Very nicel images! A reminder, if I may (as I mentioned before) for optimum image quality, the cover glass thickness becomes more critical with the 60x than with the 40x, and one should avoid a significant water layer between the cover glass and the object (basically the cover glass should be touching the object). Oliver gives an excellent, succinct, clear description here:
http://www.microbehunter.com/cover-glas ... esolution/

[JimT]

75RR, what specific info would you like to know?

Gekko, thanks for the site on cover slip thickness. You are right about the criticality of the overall thickness. Took me several tries to cut a thin enough section.

[75RR]

75RR, what specific info would you like to know?

I suppose like most of us I would like to get a dry 60x someday, so the make + NA perhaps a link to where you got it if it is new. The usual suspects.

[JimT]

Amscope obj. (I have an Amscope microscope). Achromatic and NA is 0.85 bought on Amazon. Here is the link:

http://www.amazon.com/dp/B005PPLX74/ref ... objectives

[75RR]

Amscope obj. (I have an Amscope microscope). Achromatic and NA is 0.85 bought on Amazon. Here is the link:

Many thanks

[Dale]

Hey JimT, believe it or not I'm really about to buy a scope. It has infinity plan obj's which I have read have more working
distance. I don't know the obj's NA yet, but it is the 60X with a .85 that makes me ask if I should have some thinner slips around,
perhaps #1. Do you have a source for thin slips, I can't find them. Does anyone clean their slips, or just toss them?
Dale

[vasselle]

Bonjour
Très belles photos.
C'est vraie avec objectif 60 X a sec il faut une couche mine eau entre la lamelle couvre lame et il faut faire une bonne préparation du spécimen que L'on veux observer ou photographier avec le 60 X a sec ces encore plus important avec les autres objectif.
Car ces la clé pour avoir une image claire et net.
Cordialement seb

[Dale]

Bonjour
Très belles photos.
C'est vraie avec objectif 60 X a sec il faut une couche mine eau entre la lamelle couvre lame et il faut faire une bonne préparation du spécimen que L'on veux observer ou photographier avec le 60 X a sec ces encore plus important avec les autres objectif.
Car ces la clé pour avoir une image claire et net.
Cordialement seb

I am translating.....merci beacoup

[JimT]

I clean my cover slips. The obj. is for .17 slips. Mine are .13 to .17 thickness which I bought on Amazon.

[75RR]

Does anyone clean their slips, or just toss them?

I do. I use my thumb and forefinger, skin does not scratch.
I use washing up liquid to clean them, rinse the slip well and then I wash my hands with an anticeptic.
Use creolin at the moment.

[JimT]

Dale, good luck with your purchase. BTW, my 60x is .85 NA.
Let us know after you get it and how you like it.

[mrsonchus]

Absolutely superb images, both objectives. The iodine does a good job, must get some for my kit. Thanks for the post, it's very detailed and interesting.
I've a 60x - I love it too!

[JimT]

The 60xis great for that extra bit of magnification without having to resort to the 100x OI.
I must admit, I have been using the 20x a lot as well. When 10x isn't quite enough and 40x is too much.
I think Gekko said he uses his 20x a lot also.
If you don't have one that would be my next suggestion for an upgrade.

[Dale]

I check ebay frequently, but it seems to be a rare item

[charlie g]

Congrats ,Jim T on giving us a 'shoot out' between your 40X and 60X dry objectives..yes Dale will like his Plan objectives more so than achromatic objectives...they do cost more on a new microscope.

I have a wonderful 'work horse' Nikon, Japan Plan 60X/0.85,160 tube length,0.11-0.23 ( a correction-collar objective).

I have a good Nikon,China low cost 60X/0.85, painted specs: 160/0.17 (an achro with no correction collar)

I have an extremely low cost generic China 60X/0.85, etched and black painted specs: 160/ 0.17 (an achro with no correction collar).

I have two other specialty Nikon 60X objectives...each with a correction collar to twiddle to fine adjust for the thickness of the cover slip you are useing the objective with.

I have an untried EBay purchased: Leitz Wetzlar/Germany Flourite 63X/0.85, 160/0.17 (no correction collar).

So you sort of get what you pay for: the most costly ( crazy cost often times) are the highly corrected: PlanApo objectives of high NA..and with correction collars to twiddle to adjust for coverslip variations.

The Plan objectives give crisp focus over more of an objectives field of view compared to achromatic objectives...Plan objectives with a correction collar to twiddle give subtly better images than Plan objectives with no correction collars to twiddle.

For me all are good for visual work..my eyes only look at one sweet spot on the field of view I'm observing...but for image captures...Plan objectives for me I guess (?) are better than achromatic objectives,

Get both 24X50 mm, and 22X40 coverslips...both #1, and #1.5..with these you have lots of area to observe...far from the messy borders of a wetmount slide! great microscopy with these dry objectives...as you can flip back to your lower power objectives..rather than being 'stuck' with an oil-bridged 100X objective..in a specimen study. all the best, charlie guevara

[JimT]

My more two cents. My 60X is not plan and I don't notice a difference. For visual observation I agree with CharlieG, you tend to focus on the "Sweet spot" and tweek the fine focus to bring out the extra detail. For imaging you will probably wound up stacking images anyway because the focus and DOF are so critical.

[Crater Eddie]

Nice comparison! I have often thought about getting a 60x objective, and a 20x as well. Darned Ebay sellers aren't cooperating though.
CE

[mrsonchus]

Nice comparison! I have often thought about getting a 60x objective, and a 20x as well. Darned Ebay sellers aren't cooperating though.
CE

Hi, I have a 5-objective 'scope with a 4x, 20x, 40x, 60x (dry) and 100x (oil) - I asked for the 10x to be replaced with the 60x when I bought the 'scope - it's a good selection as the 60x is much more useful to me than the 10x. I suppose if your subjects are a touch on the larger side, maybe living creatures for example, the 10x would then be a better choice? I suppose it's down to personal preference really...
I've posted some stacks of onion stomata, one of which is from a 60x objective.

For objectives of very reasonable price (both 160mm and infinite) try Brunel, they have a good selection and they're a superb company to buy from. (I don't have any connection with Brunel apart from being a very satisfied customer).

regards

[charlie g]

After this nice thread, I tested the Leitz Wetzlar/Germany Flour 63X objective..it works fine.

If you are interested in both a 20X, and 63X objective, Crater Eddy ( have I enjoyed your postings a few years back, in: www.cloudynights.com ?!) ..if you are interested in both these 160 mm tube length objectives...please contact me offlist?

all the best, charlie guevara

[Crater Eddie]

PM sent Charlie.