Mitosis in onion root tip

[Seb28]

r.jpg (287.08 KiB) Viewed 4656 times

m_1000X.jpg (431.07 KiB) Viewed 4655 times

[billbillt]

Wow Seb!.. That is wonderful!... Fantastic photos!....

BillT

[mrsonchus]

Super Seb, very nice and informative pictures! What did you do to get the tissues to this position?

[Naphthalene]

Hello Seb28 and everyone!
Great ones, so sharp chromosomes! I'm a little jealous My chromosome preps looked really crispy and weird. I guess it is DIC/Nomarsky illumination, but what is the brown stain?

[JimT]

Excellent images!

[gekko]

Exceptional images! I'm amazed at the resolution you get. What objective did you use? Thank you for posting these extraordinary pictures.

[75RR]

Amazing images. I second gekko, details please!

[vasselle]

Bonjour.
Très belles photos.
Merci pour le partage.
Cordialement Seb

[einman]

Fantastic!! Worth framing!

[Seb28]

Thank you all for your kind words.

I cut approximately 2-3mm off the tip of root.
Then I placed the root tip on a slide with 1-2 drops of 10% Hcl (muriatic acid) for 5min.
After 5min I added 1-2 drops of Hematoxylin for 5min.
Then I removed Hcl and Hematoxylin with paper towel and added just one drop of Hematoxylin for another 5min.
After that I removed Hematoxylin with paper towel, I added drop of water and cover slip,then I squash it with finger and paper towel.
I used Nikon CFN 100/1.25,and DIC technique.

[Charles]

Very nice and impressive Seb!

[gekko]

Thank you, Seb28, for the additional information. I have the same objective, but cannot get anything near that quality. As I always say, the microscopist is much more important than the microscope. Thanks again.

[Crater Eddie]

Excellent images! Thanks for describing your procedure.
CE