Soft and fluffy with lots of air pockets.
When I placed the slide on the microscope stage I noticed I had not trimmed the excess Polyfill. Photo 2
So I decided to try for a stacked 3D image using CombineZP.Photo 3 is an 11 image stack with the CombineZP Pyramid Do Stack macro.
Each change in focus was a full turn of the fine focusing wheel giving an estimated depth of the edge sample of 1.1mm.
Photo 3
The PolyFill sample did not show much in the way of birefringence on the polarizing microscope so I moved the slide to the CxL
A close look with a 10x obj. does in fact show a lot of space between the fibres.
Photo 4
To get better contrast I inserted a 16mm DarkField stop. The condenser aperture was at the 100% open position.
Photo 5
Closing the condenser aperture to 30% improves the contrast and depth of field.
Photo 6
Photos 5 and 6 are good reasons to adjust the condenser aperture and try DarkField illumination.
Details:
Photos 1 and 2.
Canon SX50 HS macro mode.
Photo 3.
Labomed LB-592
Canon 5D MkII
Infinity Plan Pol 2x obj
Photos 4, 5 and 6.
Labomed CxL
OMAX 10MP USB camera
Semi Plan 10x Achromat
Post processing: Adobe lightroom
P.S. Large images are reduced in size after Dec.17/14.
