Amoeba Arcella

[hkv]

Arcella Amoeba. DIC. 60X water immersion objective. Stack of plenty. One of my favourite amoeba image because it captures many interesting internal organelles, food, shell texture as well as pseudopods. Like a little school book illustration of an amoeba.

Cupoty_Amoeba small.jpg (105.81 KiB) Viewed 1848 times

[KurtM]

Always a pleasure to see your images. This one is breathtakingly beautiful!

[deBult]

Very well done, thanks for sharing.

[PeteM]

+1 . . . Spectacular - as are the other two postings.

[macnmotion]

Wow such an amazing image, thanks for sharing here. Did you stack this from video frames? I would assume so, since the Amoeba must have had some movement.

[hkv]

Thank you all for the kind feedback! The stack was shot using a Sony A9 so the images were shot in a rapid series, but not video. The image is sort of hand stacked or stacked in batches. I decided what features I needed to include in the image (food, edge, surface texture, nuclei, food, pseudopods, etc). Then I stacked the frames containing these features individually in Zerene. After having all sub stacks done (10 ish), I then ran Zerene again and made the final composition out of the sub stacks I did in the previous steps. Then some manual retouching in Zerene to take out artefacts or avoid cases where I had multiple features in focus and a selection was needed what to prioritise. The background is a from a stack that I shot that deliberately is way out of focus so it gets a clean look.

[macnmotion]

Thank you all for the kind feedback! The stack was shot using a Sony A9 so the images were shot in a rapid series, but not video. The image is sort of hand stacked or stacked in batches. I decided what features I needed to include in the image (food, edge, surface texture, nuclei, food, pseudopods, etc). Then I stacked the frames containing these features individually in Zerene. After having all sub stacks done (10 ish), I then ran Zerene again and made the final composition out of the sub stacks I did in the previous steps. Then some manual retouching in Zerene to take out artefacts or avoid cases where I had multiple features in focus and a selection was needed what to prioritise. The background is a from a stack that I shot that deliberately is way out of focus so it gets a clean look.

Thanks for the details. So you shoot high speed bursts while rotating the focuser smoothly, at least that's what it sounds like. Unfortunately my D750 doesn't shoot quickly enough for that, so I'm limited to stacks from 1080P video for moving things.

[Francisco]

Very nice.

[hkv]

Thank you all for the kind feedback! The stack was shot using a Sony A9 so the images were shot in a rapid series, but not video. The image is sort of hand stacked or stacked in batches. I decided what features I needed to include in the image (food, edge, surface texture, nuclei, food, pseudopods, etc). Then I stacked the frames containing these features individually in Zerene. After having all sub stacks done (10 ish), I then ran Zerene again and made the final composition out of the sub stacks I did in the previous steps. Then some manual retouching in Zerene to take out artefacts or avoid cases where I had multiple features in focus and a selection was needed what to prioritise. The background is a from a stack that I shot that deliberately is way out of focus so it gets a clean look.

Thanks for the details. So you shoot high speed bursts while rotating the focuser smoothly, at least that's what it sounds like. Unfortunately my D750 doesn't shoot quickly enough for that, so I'm limited to stacks from 1080P video for moving things.

I did not use the rapid burst mode in this case. The amoeba moved slowly enough to just use single shots and refocus. The only time I have used burst mode and focusing during burst was when photographing Vorticella.

[ImperatorRex]

Very nice, great stack.
Do you know what are the larger, ~rectangular structures at 9' and 11 O'clock?

[WWWW]

The rectangular structures are possibly the cells of the desmid: Bambusina borreri (= Bambusina brebissonii)
See: https://www.desmids.nl/maand/nederlands ... sonii.html
And of course: https://youtu.be/QxyQN2KNWb0

[hkv]

The rectangular structures are possibly the cells of the desmid: Bambusina borreri (= Bambusina brebissonii)
See: https://www.desmids.nl/maand/nederlands ... sonii.html
And of course: https://youtu.be/QxyQN2KNWb0

Looks very similar in shape, so most likely you are correct.

In another forum, a commenter made a remark that the amoeba was full of (micro)plastics. He saw two plastic barrels and a COVID-test in there. I guess amoebas eat anything that they manage to catch up. n

[ImperatorRex]

Thanks you wwww!

[Sabatini]

awesome work!