Young Copepod Darkfield
Young Copepod Darkfield
A young one.
A stack of 12 images.
AO One Ten, 10x Plan Achro, Darkfield Mask, Afocal using a Nexus 6 phone
A stack of 12 images.
AO One Ten, 10x Plan Achro, Darkfield Mask, Afocal using a Nexus 6 phone
- Attachments
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- CpDf 1-26-16 IV.jpg (24.36 KiB) Viewed 5210 times
Last edited by rnabholz on Wed Jan 27, 2016 5:33 am, edited 1 time in total.
Re: Young Copepod Darkfield
There has been some discussion recently about processing and editing photos. In case anyone was interested, I thought I would share what the photo looked like prior to any adjustments.
The image below is a stack, and while obviously the stacking software does make some changes to the image, it generally does not adjust contrast, brightness, sharpness, etc. In fact many times artifacts from the stacking introduce defects that need attention. Fortunately, on this one, the software produced a pretty clean image, leaving just some normal adjustments.
My process is pretty simple and straight forward. Typically I follow these steps.
1. Crop the image as desired
2 . Resize the cropped image to 800 pixels on the longest side
3. Make any adjustments to levels
4. Clean up any dust spots, deritus, sharpening artifacts using the Rubber Stamp tool
5. Apply modest Unsharp Mask
6. Export to Jpeg at around 80 quality.
As you can see, I cropped the image to eliminate dead space, levels were good as shot, I did have some dust specs to clean up, but nothing too onerous. Then a light unsharp mask, and that was all that was need on this one.
Rod
The image below is a stack, and while obviously the stacking software does make some changes to the image, it generally does not adjust contrast, brightness, sharpness, etc. In fact many times artifacts from the stacking introduce defects that need attention. Fortunately, on this one, the software produced a pretty clean image, leaving just some normal adjustments.
My process is pretty simple and straight forward. Typically I follow these steps.
1. Crop the image as desired
2 . Resize the cropped image to 800 pixels on the longest side
3. Make any adjustments to levels
4. Clean up any dust spots, deritus, sharpening artifacts using the Rubber Stamp tool
5. Apply modest Unsharp Mask
6. Export to Jpeg at around 80 quality.
As you can see, I cropped the image to eliminate dead space, levels were good as shot, I did have some dust specs to clean up, but nothing too onerous. Then a light unsharp mask, and that was all that was need on this one.
Rod
- Attachments
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- CpDf 1-26-16 IVraw.jpg (16.4 KiB) Viewed 5209 times
Last edited by rnabholz on Wed Jan 27, 2016 1:50 pm, edited 1 time in total.
Re: Young Copepod Darkfield
Hi Rod,
Great photo.. Personally I don't see what stacking hurts... To me, it adds to the quality of the photo...
BillT
Great photo.. Personally I don't see what stacking hurts... To me, it adds to the quality of the photo...
BillT
Re: Young Copepod Darkfield
Rod, absolutely superb image. Very beautiful colors, details, and luminosity that your darkfield images exhibit.
Re: Young Copepod Darkfield
I see no issue with stacking etc. We all the know the camera does not capture what the human eye sees anyway. Use of such software etc is simply keeping with the current state of the art.
Re: Young Copepod Darkfield
Thanks guys.
Regarding stacking and processing in general. My two cents is It all depends on what you are trying to portray with your images.
If the goal is to communicate what the view through a microscope looks like, then stacking is probably not the best way to go.
If on the other hand, if the goal is to provide the viewer with a look at what the subject might look like if we could remove the limitations of the optics, physics, and our own eyes, then I think that stacking goes a long ways towards that goal.
To the uninitiated, a stacked image may be much more compelling than an image with the normal limited depth of focus experienced at high magnification. I like to think of it as a virtual equivalent of holding an enlarged subject in your hands, and experiencing the more natural view that would provide.
Both approaches serve their purpose. Horses for Courses
Thanks,
Rod
Regarding stacking and processing in general. My two cents is It all depends on what you are trying to portray with your images.
If the goal is to communicate what the view through a microscope looks like, then stacking is probably not the best way to go.
If on the other hand, if the goal is to provide the viewer with a look at what the subject might look like if we could remove the limitations of the optics, physics, and our own eyes, then I think that stacking goes a long ways towards that goal.
To the uninitiated, a stacked image may be much more compelling than an image with the normal limited depth of focus experienced at high magnification. I like to think of it as a virtual equivalent of holding an enlarged subject in your hands, and experiencing the more natural view that would provide.
Both approaches serve their purpose. Horses for Courses
Thanks,
Rod
Re: Young Copepod Darkfield
The camera revolutionized astronomy by showing what the human eye is incapable of detecting. Photomicography does much the same work, if not quite so spectacularly. We would be remiss in not availing ourselves of all the information contained in our images by whatever means at our disposal. But what do I know?
Well, I know this: that image is lovely!
But why do you say it's a "young" one?
Well, I know this: that image is lovely!
But why do you say it's a "young" one?
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: Young Copepod Darkfield
Because he was wet behind the ears.......bah dum chsssssshKurtM wrote:Well, I know this: that image is lovely!
But why do you say it's a "young" one?
Sorry,
It was because it was relatively small. It was less than half the size of another mature individual on the slide, (Coming Soon to a forum near you...)
Re: Young Copepod Darkfield
I just saw this post ... omg...
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: Young Copepod Darkfield
Excellent images. Your Darkfield is a treat.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Young Copepod Darkfield
I've been waiting quietly giggling like a school girl for your reaction....KurtM wrote:I just saw this post ... omg...
Re: Young Copepod Darkfield
Thank you 75.75RR wrote:Excellent images. Your Darkfield is a treat.
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Re: Young Copepod Darkfield
The eye is an optic and doesn't see with the artificial depth of field that a stacked image produces. This is not so apparent with low power objectives but becomes increasingly apparent with each increase in magnification. I think stacked images and excesssively manipulated ones should be in their own section and images that have had minimal post processing should be in another section. I can't see what the big deal with such a simple separation is. It's like separating apples and oranges.
Re: Young Copepod Darkfield
Hey Phil,apochronaut wrote:The eye is an optic and doesn't see with the artificial depth of field that a stacked image produces. This is not so apparent with low power objectives but becomes increasingly apparent with each increase in magnification. I think stacked images and excesssively manipulated ones should be in their own section and images that have had minimal post processing should be in another section. I can't see what the big deal with such a simple separation is. It's like separating apples and oranges.
I understand your point, and can see your rationale.
While it is not exactly separation, I do always try to mention whether the images are a stack or a single frame. As a relative beginner, I am always trying to understand how images that appeal to me are made. Having information on the equipment used, techniques applied, and any tips is immensely helpful and goes a long way towards assisting me in identifying what I can do to improve my images.
I also try to be transparent and hope by providing the info on every posting, that people can draw their own conclusions about their own preferences.
For what it is worth....☺
Rod
Re: Young Copepod Darkfield
I found the stacked images you provided here to be superbly done...
BillT
BillT
Re: Young Copepod Darkfield
Thank you Bill.billbillt wrote:I found the stacked images you provided here to be superbly done...
BillT
Re: Young Copepod Darkfield
They are nice images and I agree with Kurt re "Wet behind the ears", OMG.
Re: Young Copepod Darkfield
Bonjour
Idem je trouve image très belle.
Et le fond noir est superbe.
Cordialement seb
Idem je trouve image très belle.
Et le fond noir est superbe.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D
Boitier EOS 1200D + EOS 1100D