Desmid

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Desmid

#1 Post by gekko » Fri Jan 29, 2016 12:34 am

Cosmarium. There is what appears to be some smearing, especially at the top of the desmid and in the diatom, that puzzles me, but it may have been due to movement caused by a nearby rotifer that was outside the field of view.

[Brightfield; 40x objective].
Image

Thanks again to Crater Eddie for the water sample.

User avatar
rnabholz
Posts: 3074
Joined: Thu Jan 08, 2015 10:11 pm
Location: Iowa USA
Contact:

Re: Desmid

#2 Post by rnabholz » Fri Jan 29, 2016 1:46 am

Beautiful colors, great sharpness, a nice seemingly serene setting.

Is this a single frame, if so the depth of focus for a 40x is very nice.

I like it a lot.

Rod

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Desmid

#3 Post by gekko » Fri Jan 29, 2016 12:01 pm

rnabholz wrote:Beautiful colors, great sharpness, a nice seemingly serene setting.
Is this a single frame, if so the depth of focus for a 40x is very nice.
I like it a lot.
Rod
Many thanks, Rod, for your too-kind comment. Yes, this is a single frame (I always say when I use a stack-- doing stacks is not a favorite activity of mine simply because, for whatever reason, my stacks usually come out very poorly :( )

User avatar
75RR
Posts: 7130
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Desmid

#4 Post by 75RR » Fri Jan 29, 2016 12:04 pm

Nice image and nice composition
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

JimT
Posts: 3247
Joined: Fri Oct 24, 2014 1:57 pm

Re: Desmid

#5 Post by JimT » Fri Jan 29, 2016 3:40 pm

Nice. Clearly something in the oblique imaging process caused the cyan in your other image.

billbillt
Posts: 2895
Joined: Mon Nov 10, 2014 10:01 pm

Re: Desmid

#6 Post by billbillt » Fri Jan 29, 2016 3:54 pm

I find this very well done..

BillT

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Desmid

#7 Post by gekko » Fri Jan 29, 2016 4:30 pm

75RR, JimT, and BillT, thank you very much!

JimT, thank you for your suggestion. I think the cyan background in the image (with grey background through the eyepieces) occurs in DIC, but not in oblique illumination. And it also seems to occur in cross-polarized image captured by the camera, and since both use polarization, I thought that perhaps that was involved somehow.

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Desmid

#8 Post by gekko » Fri Jan 29, 2016 4:38 pm

gekko wrote:
rnabholz wrote:Beautiful colors, great sharpness, a nice seemingly serene setting.
Is this a single frame, if so the depth of focus for a 40x is very nice.
I like it a lot.
Rod
Many thanks, Rod, for your too-kind comment. Yes, this is a single frame (I always say when I use a stack-- doing stacks is not a favorite activity of mine simply because, for whatever reason, my stacks usually come out very poorly :( )
Rod, I think I misunderstood your comment about depth of field: I believe in this case it was between 0.5 and 1 µm, so it is very shallow indeed. I think the image depicts a thin optical section through approximately the middle of the desmid, rather than representing it as a whole as a focus stack would.

User avatar
vasselle
Posts: 2763
Joined: Sat Oct 25, 2014 5:32 pm
Location: France

Re: Desmid

#9 Post by vasselle » Fri Jan 29, 2016 5:02 pm

Bonjour Gekko
Très belle photo.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

User avatar
rnabholz
Posts: 3074
Joined: Thu Jan 08, 2015 10:11 pm
Location: Iowa USA
Contact:

Re: Desmid

#10 Post by rnabholz » Fri Jan 29, 2016 5:03 pm

gekko wrote:
gekko wrote:
rnabholz wrote:Beautiful colors, great sharpness, a nice seemingly serene setting.
Is this a single frame, if so the depth of focus for a 40x is very nice.
I like it a lot.
Rod
Many thanks, Rod, for your too-kind comment. Yes, this is a single frame (I always say when I use a stack-- doing stacks is not a favorite activity of mine simply because, for whatever reason, my stacks usually come out very poorly :( )
Rod, I think I misunderstood your comment about depth of field: I believe in this case it was between 0.5 and 1 µm, so it is very shallow indeed. I think the image depicts a thin optical section through approximately the middle of the desmid, rather than representing it as a whole as a focus stack would.
It seemed to me to have a depth and showed a good deal of sharp detail across the face of the desmid, more than I would have expected with that objective.

Whatever the case, it is a highly successful image.

Rod

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Desmid

#11 Post by gekko » Fri Jan 29, 2016 7:55 pm

rnabholz wrote: It seemed to me to have a depth and showed a good deal of sharp detail across the face of the desmid, more than I would have expected with that objective.
Thanks again Rod. I think I can illustrate the way I think of it: let us say you place the Cosmarium in your super home-made microtome. Then you turn the bolt to expose about half the thickness, and, with your knife, cut the exposed top half off and throw it away. Then you advance the remaining part of the Cosmarium about one micrometer, and cut the 1 µm slice, and place it under your microscope. As far as I understand, that is what we are seeing in the image above. The details are on the inside of the cell, not at the top surface, so were are not seeing depth. I think. And I hope someone knowledgeable will correct me, as I'd like to know where I am wrong.

User avatar
rnabholz
Posts: 3074
Joined: Thu Jan 08, 2015 10:11 pm
Location: Iowa USA
Contact:

Re: Desmid

#12 Post by rnabholz » Fri Jan 29, 2016 8:21 pm

gekko wrote:
rnabholz wrote: It seemed to me to have a depth and showed a good deal of sharp detail across the face of the desmid, more than I would have expected with that objective.
Thanks again Rod. I think I can illustrate the way I think of it: let us say you place the Cosmarium in your super home-made microtome. Then you turn the bolt to expose about half the thickness, and, with your knife, cut the exposed top half off and throw it away. Then you advance the remaining part of the Cosmarium about one micrometer, and cut the 1 µm slice, and place it under your microscope. As far as I understand, that is what we are seeing in the image above. The details are on the inside of the cell, not at the top surface, so were are not seeing depth. I think. And I hope someone knowledgeable will correct me, as I'd like to know where I am wrong.
Thanks Gekko. I do understand the "slice" concept. Perhaps it is the various shades of color, but to my eyes it seemed to have a depth that I did not expect, and to your point did not exist.

It appears it was an illusion, but still pleasing to my eye none the less.

Rod

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Desmid

#13 Post by gekko » Fri Jan 29, 2016 10:05 pm

I apologize, Rod, for my simplistic attempt at description: it is the way my mind works.

User avatar
rnabholz
Posts: 3074
Joined: Thu Jan 08, 2015 10:11 pm
Location: Iowa USA
Contact:

Re: Desmid

#14 Post by rnabholz » Fri Jan 29, 2016 10:28 pm

gekko wrote:I apologize, Rod, for my simplistic attempt at description: it is the way my mind works.
No apology necessary! I was sure your instincts regarding your pupil's capabilities was guiding you... ;^)

Post Reply