[Brightfield; 40x objective].

Thanks again to Crater Eddie for the water sample.
Many thanks, Rod, for your too-kind comment. Yes, this is a single frame (I always say when I use a stack-- doing stacks is not a favorite activity of mine simply because, for whatever reason, my stacks usually come out very poorlyrnabholz wrote:Beautiful colors, great sharpness, a nice seemingly serene setting.
Is this a single frame, if so the depth of focus for a 40x is very nice.
I like it a lot.
Rod
Rod, I think I misunderstood your comment about depth of field: I believe in this case it was between 0.5 and 1 µm, so it is very shallow indeed. I think the image depicts a thin optical section through approximately the middle of the desmid, rather than representing it as a whole as a focus stack would.gekko wrote:Many thanks, Rod, for your too-kind comment. Yes, this is a single frame (I always say when I use a stack-- doing stacks is not a favorite activity of mine simply because, for whatever reason, my stacks usually come out very poorlyrnabholz wrote:Beautiful colors, great sharpness, a nice seemingly serene setting.
Is this a single frame, if so the depth of focus for a 40x is very nice.
I like it a lot.
Rod)
It seemed to me to have a depth and showed a good deal of sharp detail across the face of the desmid, more than I would have expected with that objective.gekko wrote:Rod, I think I misunderstood your comment about depth of field: I believe in this case it was between 0.5 and 1 µm, so it is very shallow indeed. I think the image depicts a thin optical section through approximately the middle of the desmid, rather than representing it as a whole as a focus stack would.gekko wrote:Many thanks, Rod, for your too-kind comment. Yes, this is a single frame (I always say when I use a stack-- doing stacks is not a favorite activity of mine simply because, for whatever reason, my stacks usually come out very poorlyrnabholz wrote:Beautiful colors, great sharpness, a nice seemingly serene setting.
Is this a single frame, if so the depth of focus for a 40x is very nice.
I like it a lot.
Rod)
Thanks again Rod. I think I can illustrate the way I think of it: let us say you place the Cosmarium in your super home-made microtome. Then you turn the bolt to expose about half the thickness, and, with your knife, cut the exposed top half off and throw it away. Then you advance the remaining part of the Cosmarium about one micrometer, and cut the 1 µm slice, and place it under your microscope. As far as I understand, that is what we are seeing in the image above. The details are on the inside of the cell, not at the top surface, so were are not seeing depth. I think. And I hope someone knowledgeable will correct me, as I'd like to know where I am wrong.rnabholz wrote: It seemed to me to have a depth and showed a good deal of sharp detail across the face of the desmid, more than I would have expected with that objective.
Thanks Gekko. I do understand the "slice" concept. Perhaps it is the various shades of color, but to my eyes it seemed to have a depth that I did not expect, and to your point did not exist.gekko wrote:Thanks again Rod. I think I can illustrate the way I think of it: let us say you place the Cosmarium in your super home-made microtome. Then you turn the bolt to expose about half the thickness, and, with your knife, cut the exposed top half off and throw it away. Then you advance the remaining part of the Cosmarium about one micrometer, and cut the 1 µm slice, and place it under your microscope. As far as I understand, that is what we are seeing in the image above. The details are on the inside of the cell, not at the top surface, so were are not seeing depth. I think. And I hope someone knowledgeable will correct me, as I'd like to know where I am wrong.rnabholz wrote: It seemed to me to have a depth and showed a good deal of sharp detail across the face of the desmid, more than I would have expected with that objective.
No apology necessary! I was sure your instincts regarding your pupil's capabilities was guiding you... ;^)gekko wrote:I apologize, Rod, for my simplistic attempt at description: it is the way my mind works.