Ovule and Ovaries, glorious colour, mysterious cells
Posted: Sat Feb 27, 2016 4:53 pm
Hi all, I've a few pictures of ovules of the Daffodil sections again, these are a mixture of stain-types I've tried. Also I've concentrated of the embryo early stages in particular the first couple of cell-divisions of the embryo as it begins to form...
Here's a few quick pictures, I'll try to add some info later and hopefully some more regarding what the sections are actually showing! I'm still learning this plant embryology as I go, great fascinating fun.
This is my first dalliance with my newly arrived 'Harris Haematoxylin' nuclear stain, the idea being to use this as a 'speciality' nuclear stain together with Safranin and Fast-green, two excellent & complementary stains that you've seen in many of my latest slides since I've managed to get them working together well.
Here is an attempted tri-stained ovule within the ovary (in TS) of the Daffodil seen in many of my current set of sections detailing the male (anthers and pollen) and female (ovary and ovules) reproductive processes and anatomy of a Daffodil.
This is a 39-image stitch of x100 objective pictures, I love the x100, it is really bright and always gives me excellent resolution - it's my favourite objective for sure.
It is possible to see the Fast-green in the cells to the far right, the HH is the dark-purplish of the dense cells of the ovule (nucellus tissue) and nuclei. It seems here that two cells of the actual embryo-proper (central) have each taken one stain each, one seems to have taken Harris-Haematoxylin the other is the pink of Safranin (which or course is a nuclear-stain in it's own right).. Very interesting and I would like to find out if this is accidental or indicative of a difference between these two nuclei? Here's a cropped version of the x100 stitch, the resolution remains pretty good I think, This is a stacked image, not a lot of difference in detail seen in the above cropped (single focus without stacking) image of the stitched master-pieces, Here are some cells of the nucellus, that tissue from within which the ovary begins do grow, again with the tri-stain method,
some of these cells also apper to have 'atypical' nuclei and I suspect things are happening in this tissue that I have yet to understand or recognize! Another case of 'to the books'! See what you think it all means... Finally, you may wish to shield your eyes or grab a polarizer for this one!
This is an 'enhanced' (with ammonia-water) section stained with the savagely-fast and efficient (basic) Fuchsine stain in 0.5% aqueous preparation after 5 minutes of alkali-'enhanced' staining! The idea of these tests was to achieve pure strength of staining, no subtlety was attempted (or indeed achieved! ) at these early stages - this is pretty evident in this monstrously vivid almost frighteningly bright image! This slide almost glows in the dark it's that vivid! Apologies if that last image has made your head spin!
Anyway, sorry to be so brief, I hope to post some more analytical information perhaps later tonight if I get the chance! Hope you like these and find them interesting.
Back soon.
Here's a few quick pictures, I'll try to add some info later and hopefully some more regarding what the sections are actually showing! I'm still learning this plant embryology as I go, great fascinating fun.
This is my first dalliance with my newly arrived 'Harris Haematoxylin' nuclear stain, the idea being to use this as a 'speciality' nuclear stain together with Safranin and Fast-green, two excellent & complementary stains that you've seen in many of my latest slides since I've managed to get them working together well.
Here is an attempted tri-stained ovule within the ovary (in TS) of the Daffodil seen in many of my current set of sections detailing the male (anthers and pollen) and female (ovary and ovules) reproductive processes and anatomy of a Daffodil.
This is a 39-image stitch of x100 objective pictures, I love the x100, it is really bright and always gives me excellent resolution - it's my favourite objective for sure.
It is possible to see the Fast-green in the cells to the far right, the HH is the dark-purplish of the dense cells of the ovule (nucellus tissue) and nuclei. It seems here that two cells of the actual embryo-proper (central) have each taken one stain each, one seems to have taken Harris-Haematoxylin the other is the pink of Safranin (which or course is a nuclear-stain in it's own right).. Very interesting and I would like to find out if this is accidental or indicative of a difference between these two nuclei? Here's a cropped version of the x100 stitch, the resolution remains pretty good I think, This is a stacked image, not a lot of difference in detail seen in the above cropped (single focus without stacking) image of the stitched master-pieces, Here are some cells of the nucellus, that tissue from within which the ovary begins do grow, again with the tri-stain method,
some of these cells also apper to have 'atypical' nuclei and I suspect things are happening in this tissue that I have yet to understand or recognize! Another case of 'to the books'! See what you think it all means... Finally, you may wish to shield your eyes or grab a polarizer for this one!
This is an 'enhanced' (with ammonia-water) section stained with the savagely-fast and efficient (basic) Fuchsine stain in 0.5% aqueous preparation after 5 minutes of alkali-'enhanced' staining! The idea of these tests was to achieve pure strength of staining, no subtlety was attempted (or indeed achieved! ) at these early stages - this is pretty evident in this monstrously vivid almost frighteningly bright image! This slide almost glows in the dark it's that vivid! Apologies if that last image has made your head spin!
Anyway, sorry to be so brief, I hope to post some more analytical information perhaps later tonight if I get the chance! Hope you like these and find them interesting.
Back soon.